Analysis of an acyl-CoA binding protein in Aspergillus oryzae that undergoes unconventional secretion

Hee Su Kwon, Kouhei Kawaguchi, Takashi Kikuma, Kaoru Takegawa, Katsuhiko Kitamoto, Yujiro Higuchi

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Acyl-CoA binding protein (ACBP) plays important roles in the metabolism of lipids in eukaryotic cells. In the industrially important filamentous fungus Aspergillus oryzae, although we have previously demonstrated that the A. oryzae ACBP (AoACBP) localizes to punctate structures and exhibits long-range motility, which is dependent on autophagy-related proteins, the physiological role of AoACBP remains elusive. Here, we describe identification and characterization of another ACBP from A. oryzae; we named this ACBP as AoAcb2 and accordingly renamed AoACBP as AoAcb1. The deduced amino acid sequence of AoAcb2 lacked a signal peptide. Phylogenetic analysis classified AoAcb2 into a clade that was same as the ACBP Acb1 of the model yeast Saccharomyces cerevisiae, but was different from that of AoAcb1. In contrast to punctate localization of AoAcb1, AoAcb2 was found to be dispersedly distributed in the cytoplasm, as was previously observed for the S. cerevisiae Acb1. Since we could not generate an Aoacb2 disruptant, we created an Aoacb2 conditional mutant that exhibited less growth under Aoacb2-repressed condition, suggesting that Aoacb2 is an essential gene for growth. Moreover, we observed that A. oryzae AoAcb2, but not A. oryzae AoAcb1, was secreted under carbon-starved condition, suggesting that AoAcb2 might be secreted via the unconventional protein secretion (UPS) pathway, just like S. cerevisiae Acb1. We also demonstrated that the unconventional secretion of AoAcb2 was dependent on the t-SNARE AoSso1, but was independent of the autophagy-related protein AoAtg1, suggesting that the unconventional secretion of AoAcb2, unlike that of S. cerevisiae Acb1, via the UPS pathway, is not regulated by the autophagy machinery. Thus, the filamentous fungus A. oryzae harbors two types of ACBPs, one of which appears to be essential for growth and undergoes unconventional secretion.

Original languageEnglish
Pages (from-to)481-486
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume493
Issue number1
DOIs
Publication statusPublished - Nov 4 2017

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Diazepam Binding Inhibitor
Aspergillus oryzae
Aspergillus
Yeast
Saccharomyces cerevisiae
Fungi
Proteins
Secretory Pathway
SNARE Proteins
Staphylococcal Protein A
Ports and harbors
Protein Sorting Signals
Metabolism
Growth
Machinery
Carbon
Genes
Essential Genes
Autophagy
Eukaryotic Cells

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Analysis of an acyl-CoA binding protein in Aspergillus oryzae that undergoes unconventional secretion. / Kwon, Hee Su; Kawaguchi, Kouhei; Kikuma, Takashi; Takegawa, Kaoru; Kitamoto, Katsuhiko; Higuchi, Yujiro.

In: Biochemical and Biophysical Research Communications, Vol. 493, No. 1, 04.11.2017, p. 481-486.

Research output: Contribution to journalArticle

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AU - Kitamoto, Katsuhiko

AU - Higuchi, Yujiro

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