TY - JOUR
T1 - Analysis of Capillary Electrophoresis Noise Characteristics
AU - Chen, Gong
AU - Zhu, Xi Fang
AU - Dou, Xiao Ming
AU - Liu, Chen Chen
AU - Xu, Qing Quan
AU - Yamaguchi, Yoshinori
AU - Ni, Yi
N1 - Funding Information:
The National Natural Science Foundation of China (No. 21305089), Natural Science Foundation of Jiangsu Province of China (No. BK20130245), Key Laboratory of Underwater Acoustic Communication and Marine Information Technology (Xiamen University) and Qinglan Project of Jiangsu Province.
Publisher Copyright:
© 2017, Science Press. All right reserved.
PY - 2017/6/1
Y1 - 2017/6/1
N2 - In order to optimize the fluorescence detection capillary electrophoresis system and improve the detection sensitivity, the signal noise characters were studied when separating 100~1000 base pair deoxyribonucleic acid ladder under direct current electric field in hydroxyethyl cellulose solutions. The dependence of signal noise on separation electric field intensity, polymer concentration, hydroxyethyl cellulose molecular weight, the effective length and the capillary shape were investigated. Experiment result shows that optimum parameters can be obtained under best signal noise ratio, which are the separation electric field strength of 500~600 V/cm, hydroxyethyl cellulose concentration of 0.6%~0.7%, hydroxyethyl cellulose molecular weight of 250, round inner diameter of 50 μm and effective capillary length of 8 cm.
AB - In order to optimize the fluorescence detection capillary electrophoresis system and improve the detection sensitivity, the signal noise characters were studied when separating 100~1000 base pair deoxyribonucleic acid ladder under direct current electric field in hydroxyethyl cellulose solutions. The dependence of signal noise on separation electric field intensity, polymer concentration, hydroxyethyl cellulose molecular weight, the effective length and the capillary shape were investigated. Experiment result shows that optimum parameters can be obtained under best signal noise ratio, which are the separation electric field strength of 500~600 V/cm, hydroxyethyl cellulose concentration of 0.6%~0.7%, hydroxyethyl cellulose molecular weight of 250, round inner diameter of 50 μm and effective capillary length of 8 cm.
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U2 - 10.3788/gzxb20174606.0612004
DO - 10.3788/gzxb20174606.0612004
M3 - Article
AN - SCOPUS:85026553273
SN - 1004-4213
VL - 46
JO - Guangzi Xuebao/Acta Photonica Sinica
JF - Guangzi Xuebao/Acta Photonica Sinica
IS - 6
M1 - 0612004
ER -