Analysis of CD40 ligand gene mutations in patients with primary biliary cirrhosis

M. Higuchi, T. Horiuchi, T. Kojima, H. Nishizaka, H. Ishibashi, K. Hayashi, Y. Niho, K. Nagasawa

Research output: Contribution to journalArticlepeer-review

17 Citations (Scopus)

Abstract

An elevated immunoglobulin (Ig)M concentration in serum is a common and distinctive feature of primary biliary cirrhosis (PBC). Little is known, however, about the mechanism of hyper-IgM in PBC. CD40 ligand (CD40L) has a crucial role in immunoglobulin class switching in B cells. Mutations in the gene encoding CD40L are known to induce X-linked hyper-IgM syndrome. To identify mutations in the gene for CD40L in PBC patients, we analyzed CD40L gene mutations, using reverse transcription (RT)-PCR single-strand conformation polymorphism (SSCP) analysis. No mutations were detected in cDNA from any of 24 PBC patients by the RT-PCR-SSCP technique. These data suggest that other, unidentified mechanisms are involved in hyper-IgM in PBC patients.

Original languageEnglish
Pages (from-to)429-432
Number of pages4
JournalScandinavian Journal of Clinical and Laboratory Investigation
Volume58
Issue number5
DOIs
Publication statusPublished - Nov 7 1998

All Science Journal Classification (ASJC) codes

  • Clinical Biochemistry

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