Analysis of circulating hematopoietic progenitor cells after peripheral blood stem cell transplantation

Naira Mahmut, Yoshio Katayama, Katsuto Takenaka, Takanori Teshima, Yuju Ohno, Kenji Imajyo, Masamichi Hara, Katsuji Shinagawa, Fumihiko Ishimaru, Kazuma Ikeda, Kenji Niiya, Mine Harada

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Abstract

We investigated the kinetics of posttransplant circulating progenitor cells (PTCPC) in the early phase after autologous (auto-) and allogeneic (allo-) peripheral blood stem cell transplantation (PBSCT). We analyzed the number of myeloid progenitor cells (CFU-GM) per 10 ml of peripheral blood (PB) on days 0 (just prior to transplantation), 1 (12-15 hours after completion of first transplantation), 2, 3, 5, 7, 10, 14, 17, 21 and 28 (after auto-PBSCT), and also additionally on day 35 after allo-PBSCT. A standard methylcellulose colony assay was used for analysing the number of CFU-GGM and BFU-E on all of the days. In addition, high proliferative potential-colony forming cells (HPP-CFC) of the harvested PBSC from donors and day 1 PB from recipients were assayed in 5 allo-PBSCT patients. Furthermore, a proportion of CD38- cells among CD34+ cells in the harvested PBSC and day 1 PB was evaluated by two-color flow cytometric analysis in 5 allo-PBSCT patients. The number of CFU-GM on day 1 ranged from 7 to 119 per 10 ml PB after auto-PBSCT, and from 15 to 61 per 10 ml PB after allo-PBSCT. After these transient increases, PTCPC diminished rapidly. Then, PTCPC emerged again on day 7 after auto-PBSCT and on day 10 or 14 after allo-PBSCT along with neutrophil recovery. A proportion of HPP-CFC among myeloid colonies from day 1 PB of recipients was significantly higher than that from the harvested PBSC from donors (65.6 ± 12.7% vs. 17.4 ± 13.0%, respectively, n = 5, P = 0.0013). In addition, two-color flow cytometric analysis revealed that the proportion of CD34+CD38- cells was significantly higher in day 1 PB of recipients than in the harvested PBSC from donors (57.5 ± 17.6% vs. 11.7 ± 4.9%, n = 5, P = 0.005). These observations suggest that both primitive and committed transplanted myeloid progenitor cells may circulate in the very early period following PBSCT.

Original languageEnglish
Pages (from-to)36-42
Number of pages7
JournalInternational Journal of Hematology
Volume69
Issue number1
Publication statusPublished - Dec 1 1999

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Peripheral Blood Stem Cell Transplantation
Hematopoietic Stem Cells
Myeloid Progenitor Cells
Granulocyte-Macrophage Progenitor Cells
Stem Cells
Tissue Donors
Color
Transplantation
Erythroid Precursor Cells
Methylcellulose
Myeloid Cells
Neutrophils

All Science Journal Classification (ASJC) codes

  • Hematology

Cite this

Mahmut, N., Katayama, Y., Takenaka, K., Teshima, T., Ohno, Y., Imajyo, K., ... Harada, M. (1999). Analysis of circulating hematopoietic progenitor cells after peripheral blood stem cell transplantation. International Journal of Hematology, 69(1), 36-42.

Analysis of circulating hematopoietic progenitor cells after peripheral blood stem cell transplantation. / Mahmut, Naira; Katayama, Yoshio; Takenaka, Katsuto; Teshima, Takanori; Ohno, Yuju; Imajyo, Kenji; Hara, Masamichi; Shinagawa, Katsuji; Ishimaru, Fumihiko; Ikeda, Kazuma; Niiya, Kenji; Harada, Mine.

In: International Journal of Hematology, Vol. 69, No. 1, 01.12.1999, p. 36-42.

Research output: Contribution to journalArticle

Mahmut, N, Katayama, Y, Takenaka, K, Teshima, T, Ohno, Y, Imajyo, K, Hara, M, Shinagawa, K, Ishimaru, F, Ikeda, K, Niiya, K & Harada, M 1999, 'Analysis of circulating hematopoietic progenitor cells after peripheral blood stem cell transplantation', International Journal of Hematology, vol. 69, no. 1, pp. 36-42.
Mahmut N, Katayama Y, Takenaka K, Teshima T, Ohno Y, Imajyo K et al. Analysis of circulating hematopoietic progenitor cells after peripheral blood stem cell transplantation. International Journal of Hematology. 1999 Dec 1;69(1):36-42.
Mahmut, Naira ; Katayama, Yoshio ; Takenaka, Katsuto ; Teshima, Takanori ; Ohno, Yuju ; Imajyo, Kenji ; Hara, Masamichi ; Shinagawa, Katsuji ; Ishimaru, Fumihiko ; Ikeda, Kazuma ; Niiya, Kenji ; Harada, Mine. / Analysis of circulating hematopoietic progenitor cells after peripheral blood stem cell transplantation. In: International Journal of Hematology. 1999 ; Vol. 69, No. 1. pp. 36-42.
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AU - Imajyo, Kenji

AU - Hara, Masamichi

AU - Shinagawa, Katsuji

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N2 - We investigated the kinetics of posttransplant circulating progenitor cells (PTCPC) in the early phase after autologous (auto-) and allogeneic (allo-) peripheral blood stem cell transplantation (PBSCT). We analyzed the number of myeloid progenitor cells (CFU-GM) per 10 ml of peripheral blood (PB) on days 0 (just prior to transplantation), 1 (12-15 hours after completion of first transplantation), 2, 3, 5, 7, 10, 14, 17, 21 and 28 (after auto-PBSCT), and also additionally on day 35 after allo-PBSCT. A standard methylcellulose colony assay was used for analysing the number of CFU-GGM and BFU-E on all of the days. In addition, high proliferative potential-colony forming cells (HPP-CFC) of the harvested PBSC from donors and day 1 PB from recipients were assayed in 5 allo-PBSCT patients. Furthermore, a proportion of CD38- cells among CD34+ cells in the harvested PBSC and day 1 PB was evaluated by two-color flow cytometric analysis in 5 allo-PBSCT patients. The number of CFU-GM on day 1 ranged from 7 to 119 per 10 ml PB after auto-PBSCT, and from 15 to 61 per 10 ml PB after allo-PBSCT. After these transient increases, PTCPC diminished rapidly. Then, PTCPC emerged again on day 7 after auto-PBSCT and on day 10 or 14 after allo-PBSCT along with neutrophil recovery. A proportion of HPP-CFC among myeloid colonies from day 1 PB of recipients was significantly higher than that from the harvested PBSC from donors (65.6 ± 12.7% vs. 17.4 ± 13.0%, respectively, n = 5, P = 0.0013). In addition, two-color flow cytometric analysis revealed that the proportion of CD34+CD38- cells was significantly higher in day 1 PB of recipients than in the harvested PBSC from donors (57.5 ± 17.6% vs. 11.7 ± 4.9%, n = 5, P = 0.005). These observations suggest that both primitive and committed transplanted myeloid progenitor cells may circulate in the very early period following PBSCT.

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