Analysis of Dissimilatory Sulfite Reductase and 16S rRNA Gene Fragments from Deep-Sea Hydrothermal Sites of the Suiyo Seamount, Izu-Bonin Arc, Western Pacific

Tatsunori Nakagawa, Junichiro Ishibashi, Akihiko Maruyama, Toshiro Yamanaka, Yusuke Morimoto, Hiroyuki Kimura, Tetsuro Urabe, Manabu Fukui

Research output: Contribution to journalArticle

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Abstract

This study describes the occurrence of unique dissimilatory sulfite reductase (DSR) genes at a depth of 1,380 m from the deep-sea hydrothermal vent field at the Suiyo Seamount, Izu-Bonin Arc, Western Pacific, Japan. The DSR genes were obtained from microbes that grew in a catheter-type in situ growth chamber deployed for 3 days on a vent and from the effluent water of drilled holes at 5°C and natural vent fluids at 7°C. DSR clones SUIYOdsr-A and SUIYOdsr-B were not closely related to cultivated species or environmental clones. Moreover, samples of microbial communities were examined by PCR-denaturing gradient gel electrophoresis (DGGE) analysis of the 16S rRNA gene. The sequence analysis of 16S rRNA gene fragments obtained from the vent catheter after a 3-day incubation revealed the occurrence of bacterial DGGE bands affiliated with the Aquificae and γ- and ε-Proteobacteria as well as the occurrence of archaeal phylotypes affiliated with the Thermococcales and of a unique archaeon sequence that clustered with "Nanoarchaeota." The DGGE bands obtained from drilled holes and natural vent fluids from 7 to 300°C were affiliated with the δ-Proteobacteria, genus Thiomicrospira, and Pelodictyon. The dominant DGGE bands retrieved from the effluent water of casing pipes at 3 and 4°C were closely related to phylotypes obtained from the Arctic Ocean. Our results suggest the presence of microorganisms corresponding to a unique DSR lineage not detected previously from other geothermal environments.

Original languageEnglish
Pages (from-to)393-403
Number of pages11
JournalApplied and environmental microbiology
Volume70
Issue number1
DOIs
Publication statusPublished - Jan 1 2004

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Hydrogensulfite Reductase
Denaturing Gradient Gel Electrophoresis
sulfites
sulfite
denaturing gradient gel electrophoresis
seamount
rRNA Genes
Oceans and Seas
deep sea
electrokinesis
gel
Hydrothermal Vents
ribosomal RNA
Proteobacteria
gene
Pelodictyon
catheters
Nanoarchaeota
Piscirickettsiaceae
Thermococcales

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology

Cite this

Analysis of Dissimilatory Sulfite Reductase and 16S rRNA Gene Fragments from Deep-Sea Hydrothermal Sites of the Suiyo Seamount, Izu-Bonin Arc, Western Pacific. / Nakagawa, Tatsunori; Ishibashi, Junichiro; Maruyama, Akihiko; Yamanaka, Toshiro; Morimoto, Yusuke; Kimura, Hiroyuki; Urabe, Tetsuro; Fukui, Manabu.

In: Applied and environmental microbiology, Vol. 70, No. 1, 01.01.2004, p. 393-403.

Research output: Contribution to journalArticle

Nakagawa, Tatsunori ; Ishibashi, Junichiro ; Maruyama, Akihiko ; Yamanaka, Toshiro ; Morimoto, Yusuke ; Kimura, Hiroyuki ; Urabe, Tetsuro ; Fukui, Manabu. / Analysis of Dissimilatory Sulfite Reductase and 16S rRNA Gene Fragments from Deep-Sea Hydrothermal Sites of the Suiyo Seamount, Izu-Bonin Arc, Western Pacific. In: Applied and environmental microbiology. 2004 ; Vol. 70, No. 1. pp. 393-403.
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