Analysis of genetic variation and phylogeny of the predatory bug, Pilophorus typicus, in Japan using mitochondrial gene sequences

Katsura Ito, Hiroshi Nishikawa, Takuji Shimada, Kohei Ogawa, Yukio Minamiya, Masafumi Tomoda, Kengo Nakahira, Rika Kodama, Tatsuya Fukuda, Ryo Arakawa

Research output: Contribution to journalReview article

11 Citations (Scopus)

Abstract

Pilophorus typicus (Distant) (Heteroptera: Miridae) is a predatory bug occurring in East, Southeast, and South Asia. Because the active stages of P. typicus prey on various agricultural pest insects and mites, this species is a candidate insect as an indigenous natural enemy for use in biological control programs. However, the mass releasing of introduced natural enemies into agricultural fields may incur the risk of affecting the genetic integrity of species through hybridization with a local population. To clarify the genetic characteristics of the Japanese populations of P. typicus two portions of the mitochondrial DNA, the cytochrome oxidase subunit I (COI) (534 bp) and the cytochrome B (cytB) (217 bp) genes, were sequenced for 64 individuals collected from 55 localities in a wide range of Japan. Totals of 18 and 10 haplotypes were identified for the COI and cytB sequences, respectively (25 haplotypes over regions). Phylogenetic analysis using the maximum likelihood method revealed the existence of two genetically distinct groups in P. typicus in Japan. These groups were distributed in different geographic ranges: one occurred mainly from the Pacific coastal areas of the Kii Peninsula, the Shikoku Island, and the Ryukyu Islands; whereas the other occurred from the northern Kyushu district to the Kanto and Hokuriku districts of mainland Japan. However, both haplotypes were found in a single locality of the southern coast of the Shikoku Island. COI phylogeny incorporating other Pilophorus species revealed that these groups were only recently differentiated. Therefore, use of a certain population of P. typicus across its distribution range should be done with caution because genetic hybridization may occur.

Original languageEnglish
Article number18
JournalJournal of Insect Science
Volume11
DOIs
Publication statusPublished - May 5 2011

Fingerprint

Pilophorus (Heteroptera)
Mitochondrial Genes
Electron Transport Complex IV
Phylogeny
Islands
Haplotypes
Japan
Cytochromes
nucleotide sequences
genetic variation
Insects
phylogeny
Heteroptera
cytochrome-c oxidase
Genetic Hybridization
haplotypes
Southeastern Asia
Mites
Population Characteristics
cytochromes

All Science Journal Classification (ASJC) codes

  • Insect Science

Cite this

Analysis of genetic variation and phylogeny of the predatory bug, Pilophorus typicus, in Japan using mitochondrial gene sequences. / Ito, Katsura; Nishikawa, Hiroshi; Shimada, Takuji; Ogawa, Kohei; Minamiya, Yukio; Tomoda, Masafumi; Nakahira, Kengo; Kodama, Rika; Fukuda, Tatsuya; Arakawa, Ryo.

In: Journal of Insect Science, Vol. 11, 18, 05.05.2011.

Research output: Contribution to journalReview article

Ito, K, Nishikawa, H, Shimada, T, Ogawa, K, Minamiya, Y, Tomoda, M, Nakahira, K, Kodama, R, Fukuda, T & Arakawa, R 2011, 'Analysis of genetic variation and phylogeny of the predatory bug, Pilophorus typicus, in Japan using mitochondrial gene sequences', Journal of Insect Science, vol. 11, 18. https://doi.org/10.1673/031.011.0118
Ito, Katsura ; Nishikawa, Hiroshi ; Shimada, Takuji ; Ogawa, Kohei ; Minamiya, Yukio ; Tomoda, Masafumi ; Nakahira, Kengo ; Kodama, Rika ; Fukuda, Tatsuya ; Arakawa, Ryo. / Analysis of genetic variation and phylogeny of the predatory bug, Pilophorus typicus, in Japan using mitochondrial gene sequences. In: Journal of Insect Science. 2011 ; Vol. 11.
@article{f11cdec1047f4a6db7de7325288e4f88,
title = "Analysis of genetic variation and phylogeny of the predatory bug, Pilophorus typicus, in Japan using mitochondrial gene sequences",
abstract = "Pilophorus typicus (Distant) (Heteroptera: Miridae) is a predatory bug occurring in East, Southeast, and South Asia. Because the active stages of P. typicus prey on various agricultural pest insects and mites, this species is a candidate insect as an indigenous natural enemy for use in biological control programs. However, the mass releasing of introduced natural enemies into agricultural fields may incur the risk of affecting the genetic integrity of species through hybridization with a local population. To clarify the genetic characteristics of the Japanese populations of P. typicus two portions of the mitochondrial DNA, the cytochrome oxidase subunit I (COI) (534 bp) and the cytochrome B (cytB) (217 bp) genes, were sequenced for 64 individuals collected from 55 localities in a wide range of Japan. Totals of 18 and 10 haplotypes were identified for the COI and cytB sequences, respectively (25 haplotypes over regions). Phylogenetic analysis using the maximum likelihood method revealed the existence of two genetically distinct groups in P. typicus in Japan. These groups were distributed in different geographic ranges: one occurred mainly from the Pacific coastal areas of the Kii Peninsula, the Shikoku Island, and the Ryukyu Islands; whereas the other occurred from the northern Kyushu district to the Kanto and Hokuriku districts of mainland Japan. However, both haplotypes were found in a single locality of the southern coast of the Shikoku Island. COI phylogeny incorporating other Pilophorus species revealed that these groups were only recently differentiated. Therefore, use of a certain population of P. typicus across its distribution range should be done with caution because genetic hybridization may occur.",
author = "Katsura Ito and Hiroshi Nishikawa and Takuji Shimada and Kohei Ogawa and Yukio Minamiya and Masafumi Tomoda and Kengo Nakahira and Rika Kodama and Tatsuya Fukuda and Ryo Arakawa",
year = "2011",
month = "5",
day = "5",
doi = "10.1673/031.011.0118",
language = "English",
volume = "11",
journal = "Journal of Insect Science",
issn = "1536-2442",
publisher = "Library of the University of Arizona",

}

TY - JOUR

T1 - Analysis of genetic variation and phylogeny of the predatory bug, Pilophorus typicus, in Japan using mitochondrial gene sequences

AU - Ito, Katsura

AU - Nishikawa, Hiroshi

AU - Shimada, Takuji

AU - Ogawa, Kohei

AU - Minamiya, Yukio

AU - Tomoda, Masafumi

AU - Nakahira, Kengo

AU - Kodama, Rika

AU - Fukuda, Tatsuya

AU - Arakawa, Ryo

PY - 2011/5/5

Y1 - 2011/5/5

N2 - Pilophorus typicus (Distant) (Heteroptera: Miridae) is a predatory bug occurring in East, Southeast, and South Asia. Because the active stages of P. typicus prey on various agricultural pest insects and mites, this species is a candidate insect as an indigenous natural enemy for use in biological control programs. However, the mass releasing of introduced natural enemies into agricultural fields may incur the risk of affecting the genetic integrity of species through hybridization with a local population. To clarify the genetic characteristics of the Japanese populations of P. typicus two portions of the mitochondrial DNA, the cytochrome oxidase subunit I (COI) (534 bp) and the cytochrome B (cytB) (217 bp) genes, were sequenced for 64 individuals collected from 55 localities in a wide range of Japan. Totals of 18 and 10 haplotypes were identified for the COI and cytB sequences, respectively (25 haplotypes over regions). Phylogenetic analysis using the maximum likelihood method revealed the existence of two genetically distinct groups in P. typicus in Japan. These groups were distributed in different geographic ranges: one occurred mainly from the Pacific coastal areas of the Kii Peninsula, the Shikoku Island, and the Ryukyu Islands; whereas the other occurred from the northern Kyushu district to the Kanto and Hokuriku districts of mainland Japan. However, both haplotypes were found in a single locality of the southern coast of the Shikoku Island. COI phylogeny incorporating other Pilophorus species revealed that these groups were only recently differentiated. Therefore, use of a certain population of P. typicus across its distribution range should be done with caution because genetic hybridization may occur.

AB - Pilophorus typicus (Distant) (Heteroptera: Miridae) is a predatory bug occurring in East, Southeast, and South Asia. Because the active stages of P. typicus prey on various agricultural pest insects and mites, this species is a candidate insect as an indigenous natural enemy for use in biological control programs. However, the mass releasing of introduced natural enemies into agricultural fields may incur the risk of affecting the genetic integrity of species through hybridization with a local population. To clarify the genetic characteristics of the Japanese populations of P. typicus two portions of the mitochondrial DNA, the cytochrome oxidase subunit I (COI) (534 bp) and the cytochrome B (cytB) (217 bp) genes, were sequenced for 64 individuals collected from 55 localities in a wide range of Japan. Totals of 18 and 10 haplotypes were identified for the COI and cytB sequences, respectively (25 haplotypes over regions). Phylogenetic analysis using the maximum likelihood method revealed the existence of two genetically distinct groups in P. typicus in Japan. These groups were distributed in different geographic ranges: one occurred mainly from the Pacific coastal areas of the Kii Peninsula, the Shikoku Island, and the Ryukyu Islands; whereas the other occurred from the northern Kyushu district to the Kanto and Hokuriku districts of mainland Japan. However, both haplotypes were found in a single locality of the southern coast of the Shikoku Island. COI phylogeny incorporating other Pilophorus species revealed that these groups were only recently differentiated. Therefore, use of a certain population of P. typicus across its distribution range should be done with caution because genetic hybridization may occur.

UR - http://www.scopus.com/inward/record.url?scp=79955496800&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79955496800&partnerID=8YFLogxK

U2 - 10.1673/031.011.0118

DO - 10.1673/031.011.0118

M3 - Review article

C2 - 21526929

AN - SCOPUS:79955496800

VL - 11

JO - Journal of Insect Science

JF - Journal of Insect Science

SN - 1536-2442

M1 - 18

ER -