Analysis of GPI-AP diffusion on plasma membrane of endothelial cell using photochromic fluorescent protein

Naoto Kawasaki, Tomoya Shimada, Takekazu Fukui, Keita Hamasaki, Susumu Kudo

Research output: Contribution to journalReview article

Abstract

The viscosity of the endothelial cell membrane changes during cell migration or in response to shear stress exposure. Interestingly, the degree of viscosity change differs among the various regions of the endothelial cell. These local variations in membrane viscosity may contribute to spatially specific signal transductions. It is unclear, however, whether local membrane viscosity affects to membrane protein dynamics. To address this issue, we examined membrane protein diffusion at different sites in endothelial cells. We fused the photocromic fluorescent protein Dronpa to glycosil phosphatidylinositol-anchored protein (DGGPI) which diffuses on the extracellular surface of the endothelial cell membrane. The DGGPI diffusion coefficient D was 0.165 ± 0.013 μm 2/s (mean ± SE) at 37°C. Examining different sites in the endothelial cells at 28°C (room temperature) revealed that the DGGPI diffusion coefficient at the cell edge was 27% lower than the value measured at the of cell body center.

Original languageEnglish
Pages (from-to)194-205
Number of pages12
JournalNihon Kikai Gakkai Ronbunshu, B Hen/Transactions of the Japan Society of Mechanical Engineers, Part B
Volume78
Issue number785
DOIs
Publication statusPublished - Apr 16 2012
Externally publishedYes

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Endothelial cells
Cell membranes
viscosity
membranes
proteins
Proteins
Viscosity
Membranes
diffusion coefficient
cells
Signal transduction
shear stress
Shear stress
Cells
room temperature
Temperature

All Science Journal Classification (ASJC) codes

  • Mechanical Engineering
  • Condensed Matter Physics

Cite this

Analysis of GPI-AP diffusion on plasma membrane of endothelial cell using photochromic fluorescent protein. / Kawasaki, Naoto; Shimada, Tomoya; Fukui, Takekazu; Hamasaki, Keita; Kudo, Susumu.

In: Nihon Kikai Gakkai Ronbunshu, B Hen/Transactions of the Japan Society of Mechanical Engineers, Part B, Vol. 78, No. 785, 16.04.2012, p. 194-205.

Research output: Contribution to journalReview article

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abstract = "The viscosity of the endothelial cell membrane changes during cell migration or in response to shear stress exposure. Interestingly, the degree of viscosity change differs among the various regions of the endothelial cell. These local variations in membrane viscosity may contribute to spatially specific signal transductions. It is unclear, however, whether local membrane viscosity affects to membrane protein dynamics. To address this issue, we examined membrane protein diffusion at different sites in endothelial cells. We fused the photocromic fluorescent protein Dronpa to glycosil phosphatidylinositol-anchored protein (DGGPI) which diffuses on the extracellular surface of the endothelial cell membrane. The DGGPI diffusion coefficient D was 0.165 ± 0.013 μm 2/s (mean ± SE) at 37°C. Examining different sites in the endothelial cells at 28°C (room temperature) revealed that the DGGPI diffusion coefficient at the cell edge was 27{\%} lower than the value measured at the of cell body center.",
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AU - Kudo, Susumu

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AB - The viscosity of the endothelial cell membrane changes during cell migration or in response to shear stress exposure. Interestingly, the degree of viscosity change differs among the various regions of the endothelial cell. These local variations in membrane viscosity may contribute to spatially specific signal transductions. It is unclear, however, whether local membrane viscosity affects to membrane protein dynamics. To address this issue, we examined membrane protein diffusion at different sites in endothelial cells. We fused the photocromic fluorescent protein Dronpa to glycosil phosphatidylinositol-anchored protein (DGGPI) which diffuses on the extracellular surface of the endothelial cell membrane. The DGGPI diffusion coefficient D was 0.165 ± 0.013 μm 2/s (mean ± SE) at 37°C. Examining different sites in the endothelial cells at 28°C (room temperature) revealed that the DGGPI diffusion coefficient at the cell edge was 27% lower than the value measured at the of cell body center.

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