Analysis of hematopoietic niche in the mouse embryo

Keai Sinn Tan, Nathalie Brouard, Daisuke Sugiyama

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

The development, differentiation, and maturation of hematopoietic cells are regulated by the intrinsic and extrinsic regulation. Intrinsic activity is affected by cell autonomous gene expression and extrinsic factors originate from the so-called niche surrounding the hematopoietic cells. It remains unclear why the hematopoietic sites are shifted during embryogenesis. Flow cytometry and immunohistochemistry enable us to study embryonic regulation of hematopoietic niche in the mouse embryo.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages13-27
Number of pages15
DOIs
Publication statusPublished - Jan 1 2019

Publication series

NameMethods in Molecular Biology
Volume2002
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

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All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

Cite this

Tan, K. S., Brouard, N., & Sugiyama, D. (2019). Analysis of hematopoietic niche in the mouse embryo. In Methods in Molecular Biology (pp. 13-27). (Methods in Molecular Biology; Vol. 2002). Humana Press Inc.. https://doi.org/10.1007/7651_2018_176