Analysis of hematopoietic niche in the mouse embryo

Keai Sinn Tan, Nathalie Brouard, Daisuke Sugiyama

    Research output: Chapter in Book/Report/Conference proceedingChapter

    Abstract

    The development, differentiation, and maturation of hematopoietic cells are regulated by the intrinsic and extrinsic regulation. Intrinsic activity is affected by cell autonomous gene expression and extrinsic factors originate from the so-called niche surrounding the hematopoietic cells. It remains unclear why the hematopoietic sites are shifted during embryogenesis. Flow cytometry and immunohistochemistry enable us to study embryonic regulation of hematopoietic niche in the mouse embryo.

    Original languageEnglish
    Title of host publicationMethods in Molecular Biology
    PublisherHumana Press Inc.
    Pages13-27
    Number of pages15
    DOIs
    Publication statusPublished - 2019

    Publication series

    NameMethods in Molecular Biology
    Volume2002
    ISSN (Print)1064-3745
    ISSN (Electronic)1940-6029

    All Science Journal Classification (ASJC) codes

    • Molecular Biology
    • Genetics

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