Analysis of IgG4 class switch-related molecules in IgG4-related disease

Hiroto Tsuboi, Naomi Matsuo, Mana Iizuka, Sayaka Tsuzuki, Yuya Kondo, Akihiko Tanaka, Masafumi Moriyama, Isao Matsumoto, Seiji Nakamura, Takayuki Sumida

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Abstract

Introduction: Immunoglobulin G4 (IgG4)-related disease (IgG4-RD) is a new disease entity characterized by high serum IgG4 levels, IgG4-positive plasmacytic infiltration, and fibrosis in various organs. The purpose of this study was to determine the mechanism of upregulation of IgG4 class switch recombination in IgG4-RD.Methods: We extracted RNA from peripheral blood mononuclear cells (PBMCs) of patients with IgG4-RD (n = 6), Sjögren syndrome (SS) (n = 6), and healthy controls (n = 8), from CD3-positive T cells and CD20-positive B cells sorted from PBMCs of patients with IgG4-RD (n = 3), SS (n = 4), and healthy controls (n = 4), as well as from labial salivary glands (LSGs) of patients with IgG4-RD (n = 11), SS (n = 13), and healthy controls (n = 3). The mRNA expression levels of IgG4-specific class switch-related molecules, such as Th2 cytokines (IL-4 and IL-13), Treg cytokines (IL-10 and TGF-β), and transcriptional factors (GATA3 and Foxp3) were examined with quantitative polymerase chain reaction (PCR). IgG4-nonspecific class switch-related molecules, such as CD40, CD154, BAFF, APRIL, IRF4, and AID, were also examined.Results: The expression levels of Treg cytokines (IL-10 and TGF-β) and AID were significantly higher in LSGs of IgG4-RD than in SS and the controls (P < 0.05, each). In contrast, those of CD40 and CD154 were significantly lower in PBMCs of IgG4-RD than in SS (P < 0.05, each), whereas CD40 in CD20-positive B cells and CD154 in CD3-positive T cells were comparable in the three groups.Conclusion: Overexpression of IL-10, TGF-β, and AID in LSGs might play important roles in the pathogenesis of IgG4-RD, such as IgG4-specific class-switch recombination and fibrosis. IgG4 class-switch recombination seems to be mainly upregulated in affected organs.

Original languageEnglish
Article numberR171
JournalArthritis Research and Therapy
Volume14
Issue number4
DOIs
Publication statusPublished - Jul 23 2012

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Immunoglobulin Isotypes
Immunoglobulins
Lip
Salivary Glands
Interleukin-10
Genetic Recombination
Blood Cells
Cytokines
Fibrosis
B-Lymphocytes
T-Lymphocytes
Interleukin-13
Interleukin-4
Up-Regulation

All Science Journal Classification (ASJC) codes

  • Rheumatology
  • Immunology and Allergy
  • Immunology

Cite this

Tsuboi, H., Matsuo, N., Iizuka, M., Tsuzuki, S., Kondo, Y., Tanaka, A., ... Sumida, T. (2012). Analysis of IgG4 class switch-related molecules in IgG4-related disease. Arthritis Research and Therapy, 14(4), [R171]. https://doi.org/10.1186/ar3924

Analysis of IgG4 class switch-related molecules in IgG4-related disease. / Tsuboi, Hiroto; Matsuo, Naomi; Iizuka, Mana; Tsuzuki, Sayaka; Kondo, Yuya; Tanaka, Akihiko; Moriyama, Masafumi; Matsumoto, Isao; Nakamura, Seiji; Sumida, Takayuki.

In: Arthritis Research and Therapy, Vol. 14, No. 4, R171, 23.07.2012.

Research output: Contribution to journalArticle

Tsuboi, H, Matsuo, N, Iizuka, M, Tsuzuki, S, Kondo, Y, Tanaka, A, Moriyama, M, Matsumoto, I, Nakamura, S & Sumida, T 2012, 'Analysis of IgG4 class switch-related molecules in IgG4-related disease', Arthritis Research and Therapy, vol. 14, no. 4, R171. https://doi.org/10.1186/ar3924
Tsuboi H, Matsuo N, Iizuka M, Tsuzuki S, Kondo Y, Tanaka A et al. Analysis of IgG4 class switch-related molecules in IgG4-related disease. Arthritis Research and Therapy. 2012 Jul 23;14(4). R171. https://doi.org/10.1186/ar3924
Tsuboi, Hiroto ; Matsuo, Naomi ; Iizuka, Mana ; Tsuzuki, Sayaka ; Kondo, Yuya ; Tanaka, Akihiko ; Moriyama, Masafumi ; Matsumoto, Isao ; Nakamura, Seiji ; Sumida, Takayuki. / Analysis of IgG4 class switch-related molecules in IgG4-related disease. In: Arthritis Research and Therapy. 2012 ; Vol. 14, No. 4.
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AU - Tsuboi, Hiroto

AU - Matsuo, Naomi

AU - Iizuka, Mana

AU - Tsuzuki, Sayaka

AU - Kondo, Yuya

AU - Tanaka, Akihiko

AU - Moriyama, Masafumi

AU - Matsumoto, Isao

AU - Nakamura, Seiji

AU - Sumida, Takayuki

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N2 - Introduction: Immunoglobulin G4 (IgG4)-related disease (IgG4-RD) is a new disease entity characterized by high serum IgG4 levels, IgG4-positive plasmacytic infiltration, and fibrosis in various organs. The purpose of this study was to determine the mechanism of upregulation of IgG4 class switch recombination in IgG4-RD.Methods: We extracted RNA from peripheral blood mononuclear cells (PBMCs) of patients with IgG4-RD (n = 6), Sjögren syndrome (SS) (n = 6), and healthy controls (n = 8), from CD3-positive T cells and CD20-positive B cells sorted from PBMCs of patients with IgG4-RD (n = 3), SS (n = 4), and healthy controls (n = 4), as well as from labial salivary glands (LSGs) of patients with IgG4-RD (n = 11), SS (n = 13), and healthy controls (n = 3). The mRNA expression levels of IgG4-specific class switch-related molecules, such as Th2 cytokines (IL-4 and IL-13), Treg cytokines (IL-10 and TGF-β), and transcriptional factors (GATA3 and Foxp3) were examined with quantitative polymerase chain reaction (PCR). IgG4-nonspecific class switch-related molecules, such as CD40, CD154, BAFF, APRIL, IRF4, and AID, were also examined.Results: The expression levels of Treg cytokines (IL-10 and TGF-β) and AID were significantly higher in LSGs of IgG4-RD than in SS and the controls (P < 0.05, each). In contrast, those of CD40 and CD154 were significantly lower in PBMCs of IgG4-RD than in SS (P < 0.05, each), whereas CD40 in CD20-positive B cells and CD154 in CD3-positive T cells were comparable in the three groups.Conclusion: Overexpression of IL-10, TGF-β, and AID in LSGs might play important roles in the pathogenesis of IgG4-RD, such as IgG4-specific class-switch recombination and fibrosis. IgG4 class-switch recombination seems to be mainly upregulated in affected organs.

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