Analysis of internal motions of interleukin-13 variant associated with severe bronchial asthma using 15N NMR relaxation measurements

Yuichiro Yoshida, Takatoshi Ohkuri, Chika Takeda, Ryota Kuroki, Kenji Izuhara, Taiji Imoto, Tadashi Ueda

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The single nucleotide polymorphism interleukin-13 (IL-13) R110Q is associated with severe bronchial asthma because its lower affinity leads to the augmentation of local IL-13 concentration, resulting in an increase in the signal transduction via IL-13R. Since the mutation site does not directly bind to IL-13Rα2, we carried out NMR relaxation analyses of the wild-type IL-13 and IL-13-R110Q in order to examine whether the R110Q mutation affects the internal motions in IL-13 molecules. The results showed that the internal motion in the micro- to millisecond time scale on helix D, which is suggested to be important for the interaction between IL-13 and IL-13Rα2, is increased in IL-13-R110Q compared with that in the wild-type IL-13. It therefore appears that the difference in the internal motions on helix D between the wild-type IL-13 and IL-13-R110Q may be involved in their affinity differences with IL-13Rα2.

Original languageEnglish
Pages (from-to)292-297
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume358
Issue number1
DOIs
Publication statusPublished - Jun 22 2007

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Biophysics
  • Molecular Biology

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