Analysis of internal transcribed spacer (ITS) region of Phytophthora tropicalis causing quick wilt disease of black pepper in Vietnam

Pham Ngoc Dung, Ha Viet Cuong, Nguyen van Tuat, Matsumoto Masaru

Research output: Contribution to journalArticle

Abstract

Phytophthora quick wilt is a devastating disease of black peppers in Vietnam. The internal transcribed spacer (ITS) region of the ribosomal DNA of four Phytophthora samples isolated from the diseased vines in Daknong province of the central highland part of Vietnam was Polymerase chain reaction-amplified, cloned, sequenced and characterised. Database search have showed that they are most closely related to an isolate of Phytophthora tropicalis from Taiwan. Sequence comparisons and phylogenetic analyses based on the ITS region of the four Vietnamese and other GenBank isolates of P. tropicalis and a closely related species, P. capsici, provide strong evidences that the Vietnamese isolates are all different isolates of P. tropicalis.

Original languageEnglish
Pages (from-to)842-851
Number of pages10
JournalArchives of Phytopathology and Plant Protection
Volume47
Issue number7
DOIs
Publication statusPublished - Jan 1 2014

Fingerprint

black pepper
Vietnam
internal transcribed spacers
Phytophthora
ribosomal DNA
vines
Taiwan
highlands
polymerase chain reaction
phylogeny
Phytophthora tropicalis
sampling

All Science Journal Classification (ASJC) codes

  • Agronomy and Crop Science

Cite this

Analysis of internal transcribed spacer (ITS) region of Phytophthora tropicalis causing quick wilt disease of black pepper in Vietnam. / Dung, Pham Ngoc; Cuong, Ha Viet; van Tuat, Nguyen; Masaru, Matsumoto.

In: Archives of Phytopathology and Plant Protection, Vol. 47, No. 7, 01.01.2014, p. 842-851.

Research output: Contribution to journalArticle

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