Abstract
Detection of hop bittering components, iso-α-acids, in human plasma was carried out in order to prove the ingestion of beer. Three iso-α-acids, isocohumulone, isohumulone and isoadhumulone, were extracted from plasma with isooctane under acidic condition, and submitted to high-performance liquid chromatography. The components were well separated by octadecylsilica column using a mobile phase of methanol-water (77.5:22.5) containing 85% phosphoric acid (1.7g/100ml), 10% tetraethylammonium hydroxide (1g/100ml). Since isocohumulone and isohumulone were detected in beer as major components of iso- α-acids, these components were considered to be useful indicator to prove ingestion of beer.
| Original language | English |
|---|---|
| Pages (from-to) | 130-131 |
| Number of pages | 2 |
| Journal | Japanese Journal of Forensic Toxicology |
| Volume | 17 |
| Issue number | 2 |
| Publication status | Published - Jan 1 1999 |
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All Science Journal Classification (ASJC) codes
- Pathology and Forensic Medicine
- Toxicology
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Analysis of iso-α-acids in alcoholic beverage. / Ikeda, H.; Ikeda, N.; Kudo, K.; Kage, S.
In: Japanese Journal of Forensic Toxicology, Vol. 17, No. 2, 01.01.1999, p. 130-131.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Analysis of iso-α-acids in alcoholic beverage
AU - Ikeda, H.
AU - Ikeda, N.
AU - Kudo, K.
AU - Kage, S.
PY - 1999/1/1
Y1 - 1999/1/1
N2 - Detection of hop bittering components, iso-α-acids, in human plasma was carried out in order to prove the ingestion of beer. Three iso-α-acids, isocohumulone, isohumulone and isoadhumulone, were extracted from plasma with isooctane under acidic condition, and submitted to high-performance liquid chromatography. The components were well separated by octadecylsilica column using a mobile phase of methanol-water (77.5:22.5) containing 85% phosphoric acid (1.7g/100ml), 10% tetraethylammonium hydroxide (1g/100ml). Since isocohumulone and isohumulone were detected in beer as major components of iso- α-acids, these components were considered to be useful indicator to prove ingestion of beer.
AB - Detection of hop bittering components, iso-α-acids, in human plasma was carried out in order to prove the ingestion of beer. Three iso-α-acids, isocohumulone, isohumulone and isoadhumulone, were extracted from plasma with isooctane under acidic condition, and submitted to high-performance liquid chromatography. The components were well separated by octadecylsilica column using a mobile phase of methanol-water (77.5:22.5) containing 85% phosphoric acid (1.7g/100ml), 10% tetraethylammonium hydroxide (1g/100ml). Since isocohumulone and isohumulone were detected in beer as major components of iso- α-acids, these components were considered to be useful indicator to prove ingestion of beer.
UR - http://www.scopus.com/inward/record.url?scp=0032817477&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032817477&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:0032817477
VL - 17
SP - 130
EP - 131
JO - Forensic Toxicology
JF - Forensic Toxicology
SN - 1860-8965
IS - 2
ER -