Analysis of iso-α-acids in alcoholic beverage

H. Ikeda, Noriaki Ikeda, Keiko Kudo, S. Kage

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Detection of hop bittering components, iso-α-acids, in human plasma was carried out in order to prove the ingestion of beer. Three iso-α-acids, isocohumulone, isohumulone and isoadhumulone, were extracted from plasma with isooctane under acidic condition, and submitted to high-performance liquid chromatography. The components were well separated by octadecylsilica column using a mobile phase of methanol-water (77.5:22.5) containing 85% phosphoric acid (1.7g/100ml), 10% tetraethylammonium hydroxide (1g/100ml). Since isocohumulone and isohumulone were detected in beer as major components of iso- α-acids, these components were considered to be useful indicator to prove ingestion of beer.

Original languageEnglish
Pages (from-to)130-131
Number of pages2
JournalJapanese Journal of Forensic Toxicology
Volume17
Issue number2
Publication statusPublished - Jan 1 1999

Fingerprint

Beer
Alcoholic Beverages
Acids
Eating
Plasma (human)
Humulus
Tetraethylammonium
High performance liquid chromatography
Methanol
High Pressure Liquid Chromatography
Plasmas
Water
Alcoholic beverages
isohumulone
isocohumulone

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Toxicology

Cite this

Analysis of iso-α-acids in alcoholic beverage. / Ikeda, H.; Ikeda, Noriaki; Kudo, Keiko; Kage, S.

In: Japanese Journal of Forensic Toxicology, Vol. 17, No. 2, 01.01.1999, p. 130-131.

Research output: Contribution to journalArticle

Ikeda, H, Ikeda, N, Kudo, K & Kage, S 1999, 'Analysis of iso-α-acids in alcoholic beverage', Japanese Journal of Forensic Toxicology, vol. 17, no. 2, pp. 130-131.
Ikeda, H. ; Ikeda, Noriaki ; Kudo, Keiko ; Kage, S. / Analysis of iso-α-acids in alcoholic beverage. In: Japanese Journal of Forensic Toxicology. 1999 ; Vol. 17, No. 2. pp. 130-131.
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