Abstract
Detection of hop bittering components, iso-α-acids, in human plasma was carried out in order to prove the ingestion of beer. Three iso-α-acids, isocohumulone, isohumulone and isoadhumulone, were extracted from plasma with isooctane under acidic condition, and submitted to high-performance liquid chromatography. The components were well separated by octadecylsilica column using a mobile phase of methanol-water (77.5:22.5) containing 85% phosphoric acid (1.7g/100ml), 10% tetraethylammonium hydroxide (1g/100ml). Since isocohumulone and isohumulone were detected in beer as major components of iso- α-acids, these components were considered to be useful indicator to prove ingestion of beer.
Original language | English |
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Pages (from-to) | 130-131 |
Number of pages | 2 |
Journal | Japanese Journal of Forensic Toxicology |
Volume | 17 |
Issue number | 2 |
Publication status | Published - Jan 1 1999 |
All Science Journal Classification (ASJC) codes
- Pathology and Forensic Medicine
- Toxicology