Analysis of molecular diversity in water chestnut based on RAPD markers

A. Hoque, T. Anai, S. Arima

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

Molecular characterization and genetic variation patterns between 18 varieties of water chestnut (Trapa sp.) were investigated through RAPD markers. PCR amplifiable DNA was isolated using the CTAB method and amplified fragments were obtained from 20 random 10-mer and 20 random 12-mer primers. Genetic distances among the different varieties were analyzed with a UPGMA-derived dendrogram. Similarity matrix showed the similarities between varieties ranged from 0.25 to 1.0. The dendrogram results suggested that the European varieties were in the same cluster group. Japanese varieties were divided into three clusters group. Indian varieties were in the separate cluster group. Korean and Japanese small were in the same cluster group. Chinese 1-7 and Japanese medium and Kobe-large were in the same group but have sub-group. Genetic distance of Kobe small is high and most devient from other varieties and made separate cluster group.

Original languageEnglish
Pages (from-to)144-148
Number of pages5
JournalBiotechnology
Volume4
Issue number2
DOIs
Publication statusPublished - 2005
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

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