Analysis of MT1-MMP and MMP2 expression in human gastric cancers

Masaki Mori, Koshi Mimori, Takeshi Shiraishi, Tatsuo Fujie, Kinya Baba, Hiroki Kusumoto, Masaru Haraguchi, Hiroaki Ueo, Tsuyoshi Akiyoshi

Research output: Contribution to journalArticlepeer-review

95 Citations (Scopus)

Abstract

Membrane-type 1 matrix metalloproteinase (MT1-MMP) is a presumed activator of MMP2, which is one of the major proteinases in tumor cell invasion. In this study, we determined the clinico-pathologic significance of MT1-MMP expression in 68 human gastric carcinomas. The tumor-normal ratio (T/N ratio) of MT1-MMP expression was determined by reverse transcription- polymerase chain reaction analysis. To visualize the localization of MT1- MMP, an immunohistochemical study was performed. In addition, a gelatin zymography was done to examine the activation ratio of MMP2, and a correlation between MT1-MMP expression and activation of MMP2 was studied. The expression of MT1-MMP mRNA was higher in tumor tissue than in corresponding normal tissue in most cases. The mean value of the T/N ratio was 4.8. Twenty cases with T/N ≤ 4.8 showed significantly deeper invasion and higher frequency of lymph node metastasis than 48 cases with T/N < 4,8. MT1-MMP expression was an independent factor influencing both tumor invasion of the gastric wall and lymph node metastasis. Although MT1-MMP expression was not an independent prognostic factor, the patients with T/N ≤ 4.8 showed a significantly worse prognosis than those with T/N < 4.8. An immunohistochemical study demonstrated that MT1-MMP expression was mainly recognized in the tumor cells. There was a significant correlation between MT1-MMP expression and activation of MMP2. Our findings suggest that: 1) the expression of MT1-MMP may influence prognosis via tumor invasion of the gastric wall and lymph node metastasis, and 2) MT1-MMP activation of MMP2 may be clinically relevant in gastric carcinoma tumors.

Original languageEnglish
Pages (from-to)316-321
Number of pages6
JournalInternational Journal of Cancer
Volume74
Issue number3
DOIs
Publication statusPublished - 1997

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

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