Analysis of NMR spectra of sugar chains of glycolipids by multiple relayed COSY and 2D homonuclear. Hartman-Hahn spectroscopy

F. Inagaki, D. Kohda, C. Kodama, A. Suzuki

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

We applied multiple relayed COSY and 2D homonuclear Hartman-Hahn spectroscopy to globoside, a glycolipid purified from human red blood cells. The subspectra corresponding to individual sugar components were extracted even from overlapping proton resonances by taking the cross sections of 2D spectra parallel to the F2 axis at anomeric proton resonances, so that unambiguous assignments of sugar proton resonances were accomplished.

Original languageEnglish
Pages (from-to)91-97
Number of pages7
JournalFEBS Letters
Volume212
Issue number1
DOIs
Publication statusPublished - Feb 9 1987
Externally publishedYes

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Glycolipids
Sugars
Protons
Spectrum Analysis
Nuclear magnetic resonance
Spectroscopy
Globosides
Blood
Erythrocytes
Cells

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Analysis of NMR spectra of sugar chains of glycolipids by multiple relayed COSY and 2D homonuclear. Hartman-Hahn spectroscopy. / Inagaki, F.; Kohda, D.; Kodama, C.; Suzuki, A.

In: FEBS Letters, Vol. 212, No. 1, 09.02.1987, p. 91-97.

Research output: Contribution to journalArticle

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