Analysis of plasmalogen synthesis in cultured cells

Masanori Honsho, Yukio Fujiki

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Plasmalogen synthesis can be analyzed by metabolic labeling, followed by the separation of ethanolamine plasmalogens from glycerophospholipids on one-dimensional thin-layer chromatography. The vinyl-ether bond of plasmalogens is acid-labile, which allows separating plasmalogens as 2-acyl-glycerophospholipids from diacyl-glycerophospholipids.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages55-61
Number of pages7
DOIs
Publication statusPublished - Jan 1 2017

Publication series

NameMethods in Molecular Biology
Volume1595
ISSN (Print)1064-3745

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

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    Honsho, M., & Fujiki, Y. (2017). Analysis of plasmalogen synthesis in cultured cells. In Methods in Molecular Biology (pp. 55-61). (Methods in Molecular Biology; Vol. 1595). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-6937-1_6