Analysis of protein dynamics during cytokinesis in budding yeast

Satoshi Okada, C. Wloka, E. Bi

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Cytokinesis is essential for development and survival of all organisms by increasing cell number and diversity. It is a highly regulated process that requires spatiotemporal coordination of hundreds of proteins functioning in the assembly, constriction, and disassembly of a contractile actomyosin ring, targeted vesicle fusion, and localized extracellular matrix remodeling. Cytokinesis has been studied in multiple systems with a wide range of technologies to learn the common principles. In this chapter, we describe the analysis of protein dynamics during cytokinesis in the budding yeast. Saccharomyces cerevisiae by several live-cell imaging methods. This, in combination with the power of yeast genetics, has yielded novel insights into the mechanism of cytokinesis. Similar approaches are increasingly used to study this fundamental process in more complex systems.

Original languageEnglish
JournalMethods in Cell Biology
DOIs
Publication statusAccepted/In press - 2017

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Saccharomycetales
Cytokinesis
Proteins
Actomyosin
Constriction
Extracellular Matrix
Saccharomyces cerevisiae
Cell Count
Yeasts
Technology

All Science Journal Classification (ASJC) codes

  • Cell Biology

Cite this

Analysis of protein dynamics during cytokinesis in budding yeast. / Okada, Satoshi; Wloka, C.; Bi, E.

In: Methods in Cell Biology, 2017.

Research output: Contribution to journalArticle

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