Analysis of protein interactions with two-hybrid system in cultured insect cells

Hiroaki Mon, Ryohei Sugahara, Sun Mee Hong, Jae Man Lee, Yusuke Kamachi, Yutaka Kawaguchi, Takahiro Kusakabe

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)

Abstract

Many biological processes are usually coupled to the formation of protein complexes. The yeast two-hybrid system is a powerful tool for analyzing protein-protein interactions. Different patterns of protein modifications, such as glycosylation, phosphorylation, and acetylation, may affect the ability of proteins to interact. In this study, we developed the two-hybrid system that can be used in insect cells. To validate the insect two-hybrid (I2H) system, we analyzed and confirmed the known oligomer or dimer formation of silkworm Rad51 or RPA2-RPA3, respectively. The results established the feasibility of the I2H system for efficient analysis of protein interaction under conditions that closely reflect the normal physiological environment.

Original languageEnglish
Pages (from-to)180-182
Number of pages3
JournalAnalytical Biochemistry
Volume392
Issue number2
DOIs
Publication statusPublished - Sep 15 2009

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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