Analysis of protein-protein interactions on PMMA matrix

Yuya Nagasaka, Hisao Matsuno, Kimio Kurita, Takeshi Serizawa

Research output: Contribution to conferencePaperpeer-review

Abstract

Isotactic (it) and syndiotactic (st) poly(methyl methacrylate)s (PMMAs) form double-stranded helical stereocomplexes in organic solvents. We prepared PMMA stereocomplex films by a layer-by-layer assembly method, and the films were coated with protein A. Antibodies were deposited on the films, and then the binding of antigen were analyzed. Immobilized amount of protein A and Anti-HSA on stereocomplex films were greater than those on individual spin-coated films and gold substrates. When the concentration of HSAwas 0.1 μM, HSA binding amounts on stereocomplex films coated with antibodies were ten times greater than those on spin-coated PMMA films and gold substrates.

Original languageEnglish
Number of pages1
Publication statusPublished - Dec 1 2005
Externally publishedYes
Event54th SPSJ Annual Meeting 2005 - Yokohama, Japan
Duration: May 25 2005May 27 2005

Other

Other54th SPSJ Annual Meeting 2005
CountryJapan
CityYokohama
Period5/25/055/27/05

All Science Journal Classification (ASJC) codes

  • Engineering(all)

Fingerprint Dive into the research topics of 'Analysis of protein-protein interactions on PMMA matrix'. Together they form a unique fingerprint.

Cite this