Abstract
Isotactic (it) and syndiotactic (st) poly(methyl methacrylate)s (PMMAs) form double-stranded helical stereocomplexes in organic solvents. We prepared PMMA stereocomplex films by a layer-by-layer assembly method, and the films were coated with protein A. Antibodies were deposited on the films, and then the binding of antigen were analyzed. Immobilized amount of protein A and Anti-HSA on stereocomplex films were greater than those on individual spin-coated films and gold substrates. When the concentration of HSAwas 0.1 μM, HSA binding amounts on stereocomplex films coated with antibodies were ten times greater than those on spin-coated PMMA films and gold substrates.
| Original language | English |
|---|---|
| Number of pages | 1 |
| Publication status | Published - Dec 1 2005 |
| Externally published | Yes |
| Event | 54th SPSJ Annual Meeting 2005 - Yokohama, Japan Duration: May 25 2005 → May 27 2005 |
Other
| Other | 54th SPSJ Annual Meeting 2005 |
|---|---|
| Country | Japan |
| City | Yokohama |
| Period | 5/25/05 → 5/27/05 |
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All Science Journal Classification (ASJC) codes
- Engineering(all)
Cite this
Analysis of protein-protein interactions on PMMA matrix. / Nagasaka, Yuya; Matsuno, Hisao; Kurita, Kimio; Serizawa, Takeshi.
2005. Paper presented at 54th SPSJ Annual Meeting 2005, Yokohama, Japan.Research output: Contribution to conference › Paper
}
TY - CONF
T1 - Analysis of protein-protein interactions on PMMA matrix
AU - Nagasaka, Yuya
AU - Matsuno, Hisao
AU - Kurita, Kimio
AU - Serizawa, Takeshi
PY - 2005/12/1
Y1 - 2005/12/1
N2 - Isotactic (it) and syndiotactic (st) poly(methyl methacrylate)s (PMMAs) form double-stranded helical stereocomplexes in organic solvents. We prepared PMMA stereocomplex films by a layer-by-layer assembly method, and the films were coated with protein A. Antibodies were deposited on the films, and then the binding of antigen were analyzed. Immobilized amount of protein A and Anti-HSA on stereocomplex films were greater than those on individual spin-coated films and gold substrates. When the concentration of HSAwas 0.1 μM, HSA binding amounts on stereocomplex films coated with antibodies were ten times greater than those on spin-coated PMMA films and gold substrates.
AB - Isotactic (it) and syndiotactic (st) poly(methyl methacrylate)s (PMMAs) form double-stranded helical stereocomplexes in organic solvents. We prepared PMMA stereocomplex films by a layer-by-layer assembly method, and the films were coated with protein A. Antibodies were deposited on the films, and then the binding of antigen were analyzed. Immobilized amount of protein A and Anti-HSA on stereocomplex films were greater than those on individual spin-coated films and gold substrates. When the concentration of HSAwas 0.1 μM, HSA binding amounts on stereocomplex films coated with antibodies were ten times greater than those on spin-coated PMMA films and gold substrates.
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M3 - Paper
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