Analysis of protein-protein interactions on PMMA matrix

Yuya Nagasaka; Hisao Matsuno; Kimio Kurita; Takeshi Serizawa

Analysis of protein-protein interactions on PMMA matrix

Yuya Nagasaka, Hisao Matsuno, Kimio Kurita, Takeshi Serizawa

Research output: Contribution to conference › Paper › peer-review

Abstract

Isotactic (it) and syndiotactic (st) poly(methyl methacrylate)s (PMMAs) form double-stranded helical stereocomplexes in organic solvents. We prepared PMMA stereocomplex films by a layer-by-layer assembly method, and the films were coated with protein A. Antibodies were deposited on the films, and then the binding of antigen were analyzed. Immobilized amount of protein A and Anti-HSA on stereocomplex films were greater than those on individual spin-coated films and gold substrates. When the concentration of HSAwas 0.1 μM, HSA binding amounts on stereocomplex films coated with antibodies were ten times greater than those on spin-coated PMMA films and gold substrates.

Original language	English
Number of pages	1
Publication status	Published - Dec 1 2005
Externally published	Yes
Event	54th SPSJ Annual Meeting 2005 - Yokohama, Japan Duration: May 25 2005 → May 27 2005

Other

Other	54th SPSJ Annual Meeting 2005
Country	Japan
City	Yokohama
Period	5/25/05 → 5/27/05

All Science Journal Classification (ASJC) codes

Engineering(all)

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@conference{b075603dd8b2480cadb618aed72a0975,

title = "Analysis of protein-protein interactions on PMMA matrix",

abstract = "Isotactic (it) and syndiotactic (st) poly(methyl methacrylate)s (PMMAs) form double-stranded helical stereocomplexes in organic solvents. We prepared PMMA stereocomplex films by a layer-by-layer assembly method, and the films were coated with protein A. Antibodies were deposited on the films, and then the binding of antigen were analyzed. Immobilized amount of protein A and Anti-HSA on stereocomplex films were greater than those on individual spin-coated films and gold substrates. When the concentration of HSAwas 0.1 μM, HSA binding amounts on stereocomplex films coated with antibodies were ten times greater than those on spin-coated PMMA films and gold substrates.",

author = "Yuya Nagasaka and Hisao Matsuno and Kimio Kurita and Takeshi Serizawa",

year = "2005",

month = dec,

day = "1",

language = "English",

note = "54th SPSJ Annual Meeting 2005 ; Conference date: 25-05-2005 Through 27-05-2005",

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TY - CONF

T1 - Analysis of protein-protein interactions on PMMA matrix

AU - Nagasaka, Yuya

AU - Matsuno, Hisao

AU - Kurita, Kimio

AU - Serizawa, Takeshi

PY - 2005/12/1

Y1 - 2005/12/1

N2 - Isotactic (it) and syndiotactic (st) poly(methyl methacrylate)s (PMMAs) form double-stranded helical stereocomplexes in organic solvents. We prepared PMMA stereocomplex films by a layer-by-layer assembly method, and the films were coated with protein A. Antibodies were deposited on the films, and then the binding of antigen were analyzed. Immobilized amount of protein A and Anti-HSA on stereocomplex films were greater than those on individual spin-coated films and gold substrates. When the concentration of HSAwas 0.1 μM, HSA binding amounts on stereocomplex films coated with antibodies were ten times greater than those on spin-coated PMMA films and gold substrates.

AB - Isotactic (it) and syndiotactic (st) poly(methyl methacrylate)s (PMMAs) form double-stranded helical stereocomplexes in organic solvents. We prepared PMMA stereocomplex films by a layer-by-layer assembly method, and the films were coated with protein A. Antibodies were deposited on the films, and then the binding of antigen were analyzed. Immobilized amount of protein A and Anti-HSA on stereocomplex films were greater than those on individual spin-coated films and gold substrates. When the concentration of HSAwas 0.1 μM, HSA binding amounts on stereocomplex films coated with antibodies were ten times greater than those on spin-coated PMMA films and gold substrates.

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M3 - Paper

AN - SCOPUS:33645644077

T2 - 54th SPSJ Annual Meeting 2005

Y2 - 25 May 2005 through 27 May 2005

ER -