Analysis of protein-protein interactions on PMMA matrix

Yuya Nagasaka, Hisao Matsuno, Kimio Kurita, Takeshi Serizawa

Research output: Contribution to conferencePaper

Abstract

Isotactic (it) and syndiotactic (st) poly(methyl methacrylate)s (PMMAs) form double-stranded helical stereocomplexes in organic solvents. We prepared PMMA stereocomplex films by a layer-by-layer assembly method, and the films were coated with protein A. Antibodies were deposited on the films, and then the binding of antigen were analyzed. Immobilized amount of protein A and Anti-HSA on stereocomplex films were greater than those on individual spin-coated films and gold substrates. When the concentration of HSAwas 0.1 μM, HSA binding amounts on stereocomplex films coated with antibodies were ten times greater than those on spin-coated PMMA films and gold substrates.

Original languageEnglish
Number of pages1
Publication statusPublished - Dec 1 2005
Externally publishedYes
Event54th SPSJ Annual Meeting 2005 - Yokohama, Japan
Duration: May 25 2005May 27 2005

Other

Other54th SPSJ Annual Meeting 2005
CountryJapan
CityYokohama
Period5/25/055/27/05

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Polymethyl methacrylates
Proteins
Antibodies
Gold
Substrates
Antigens
Organic solvents

All Science Journal Classification (ASJC) codes

  • Engineering(all)

Cite this

Nagasaka, Y., Matsuno, H., Kurita, K., & Serizawa, T. (2005). Analysis of protein-protein interactions on PMMA matrix. Paper presented at 54th SPSJ Annual Meeting 2005, Yokohama, Japan.

Analysis of protein-protein interactions on PMMA matrix. / Nagasaka, Yuya; Matsuno, Hisao; Kurita, Kimio; Serizawa, Takeshi.

2005. Paper presented at 54th SPSJ Annual Meeting 2005, Yokohama, Japan.

Research output: Contribution to conferencePaper

Nagasaka, Y, Matsuno, H, Kurita, K & Serizawa, T 2005, 'Analysis of protein-protein interactions on PMMA matrix', Paper presented at 54th SPSJ Annual Meeting 2005, Yokohama, Japan, 5/25/05 - 5/27/05.
Nagasaka Y, Matsuno H, Kurita K, Serizawa T. Analysis of protein-protein interactions on PMMA matrix. 2005. Paper presented at 54th SPSJ Annual Meeting 2005, Yokohama, Japan.
Nagasaka, Yuya ; Matsuno, Hisao ; Kurita, Kimio ; Serizawa, Takeshi. / Analysis of protein-protein interactions on PMMA matrix. Paper presented at 54th SPSJ Annual Meeting 2005, Yokohama, Japan.1 p.
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