Analysis of proteins in urinary tract stones and urine of urolithic patients

Kayo Yokomizoi, Aki Nakayama, Eisaku Hokazono, Akiko Ninomiya, Ruriko Miyake, Nobuo Hiratsuka, Mitsuhiko Okuyama, Yuji Kato, Shizuko Kobayashi, Yoshihisa Ito, Kiyoko Shiba

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3 Citations (Scopus)

Abstract

In order to investigate the mechanism of urinary tract stone formation, we analyzed protein components in urine and the stone. Urinary proteins of healthy subjects and urolithic patients as well as protein components urinary tract stone of the urolithic patients were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Electrophoretic patterns of urinary proteins of the patients differed from those of healthy subjects after separating protein patterns into those larger than 66kDa or smaller than 30kDa. Protein constituents of urinary tract stone were mainly separated into 18 bands ranging from 26.8 to 143 kDa. Major bands among these 18 bands differed among stones from different patients. On western blotting, the developed intensities of Tamm-Horsfall protein (THP) were fainter than those of healthy subjects. Whereas intensities of albumin (ALB) were stronger than those of healthy subjects. Moreover, blotting patterns of THP of the patients on non-reducing SDS-PAGE were obviously broad. Thus, we suggest that analysis of fractionated urinary proteins or protein components of urinary tract stone may provide a tool for monitoring the prognosis or relapse in the patients.

Original languageEnglish
Pages (from-to)1109-1115
Number of pages7
JournalRinsho byori. The Japanese journal of clinical pathology
Volume53
Issue number12
Publication statusPublished - Jan 1 2005
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Medicine(all)

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    Yokomizoi, K., Nakayama, A., Hokazono, E., Ninomiya, A., Miyake, R., Hiratsuka, N., Okuyama, M., Kato, Y., Kobayashi, S., Ito, Y., & Shiba, K. (2005). Analysis of proteins in urinary tract stones and urine of urolithic patients. Rinsho byori. The Japanese journal of clinical pathology, 53(12), 1109-1115.