Analysis of proteins in urinary tract stones and urine of urolithic patients

Kayo Yokomizoi, Aki Nakayama, Eisaku Hokazono, Akiko Ninomiya, Ruriko Miyake, Nobuo Hiratsuka, Mitsuhiko Okuyama, Yuji Kato, Shizuko Kobayashi, Yoshihisa Ito, Kiyoko Shiba

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

In order to investigate the mechanism of urinary tract stone formation, we analyzed protein components in urine and the stone. Urinary proteins of healthy subjects and urolithic patients as well as protein components urinary tract stone of the urolithic patients were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Electrophoretic patterns of urinary proteins of the patients differed from those of healthy subjects after separating protein patterns into those larger than 66kDa or smaller than 30kDa. Protein constituents of urinary tract stone were mainly separated into 18 bands ranging from 26.8 to 143 kDa. Major bands among these 18 bands differed among stones from different patients. On western blotting, the developed intensities of Tamm-Horsfall protein (THP) were fainter than those of healthy subjects. Whereas intensities of albumin (ALB) were stronger than those of healthy subjects. Moreover, blotting patterns of THP of the patients on non-reducing SDS-PAGE were obviously broad. Thus, we suggest that analysis of fractionated urinary proteins or protein components of urinary tract stone may provide a tool for monitoring the prognosis or relapse in the patients.

Original languageEnglish
Pages (from-to)1109-1115
Number of pages7
JournalRinsho byori. The Japanese journal of clinical pathology
Volume53
Issue number12
Publication statusPublished - Jan 1 2005
Externally publishedYes

Fingerprint

Urinary Calculi
Urine
Healthy Volunteers
Proteins
Uromodulin
Sodium Dodecyl Sulfate
Polyacrylamide Gel Electrophoresis
Albumins
Western Blotting
Recurrence

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

Yokomizoi, K., Nakayama, A., Hokazono, E., Ninomiya, A., Miyake, R., Hiratsuka, N., ... Shiba, K. (2005). Analysis of proteins in urinary tract stones and urine of urolithic patients. Rinsho byori. The Japanese journal of clinical pathology, 53(12), 1109-1115.

Analysis of proteins in urinary tract stones and urine of urolithic patients. / Yokomizoi, Kayo; Nakayama, Aki; Hokazono, Eisaku; Ninomiya, Akiko; Miyake, Ruriko; Hiratsuka, Nobuo; Okuyama, Mitsuhiko; Kato, Yuji; Kobayashi, Shizuko; Ito, Yoshihisa; Shiba, Kiyoko.

In: Rinsho byori. The Japanese journal of clinical pathology, Vol. 53, No. 12, 01.01.2005, p. 1109-1115.

Research output: Contribution to journalArticle

Yokomizoi, K, Nakayama, A, Hokazono, E, Ninomiya, A, Miyake, R, Hiratsuka, N, Okuyama, M, Kato, Y, Kobayashi, S, Ito, Y & Shiba, K 2005, 'Analysis of proteins in urinary tract stones and urine of urolithic patients', Rinsho byori. The Japanese journal of clinical pathology, vol. 53, no. 12, pp. 1109-1115.
Yokomizoi K, Nakayama A, Hokazono E, Ninomiya A, Miyake R, Hiratsuka N et al. Analysis of proteins in urinary tract stones and urine of urolithic patients. Rinsho byori. The Japanese journal of clinical pathology. 2005 Jan 1;53(12):1109-1115.
Yokomizoi, Kayo ; Nakayama, Aki ; Hokazono, Eisaku ; Ninomiya, Akiko ; Miyake, Ruriko ; Hiratsuka, Nobuo ; Okuyama, Mitsuhiko ; Kato, Yuji ; Kobayashi, Shizuko ; Ito, Yoshihisa ; Shiba, Kiyoko. / Analysis of proteins in urinary tract stones and urine of urolithic patients. In: Rinsho byori. The Japanese journal of clinical pathology. 2005 ; Vol. 53, No. 12. pp. 1109-1115.
@article{3e4c6ff4f5a8478bb1f3daf6e62dbc95,
title = "Analysis of proteins in urinary tract stones and urine of urolithic patients",
abstract = "In order to investigate the mechanism of urinary tract stone formation, we analyzed protein components in urine and the stone. Urinary proteins of healthy subjects and urolithic patients as well as protein components urinary tract stone of the urolithic patients were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Electrophoretic patterns of urinary proteins of the patients differed from those of healthy subjects after separating protein patterns into those larger than 66kDa or smaller than 30kDa. Protein constituents of urinary tract stone were mainly separated into 18 bands ranging from 26.8 to 143 kDa. Major bands among these 18 bands differed among stones from different patients. On western blotting, the developed intensities of Tamm-Horsfall protein (THP) were fainter than those of healthy subjects. Whereas intensities of albumin (ALB) were stronger than those of healthy subjects. Moreover, blotting patterns of THP of the patients on non-reducing SDS-PAGE were obviously broad. Thus, we suggest that analysis of fractionated urinary proteins or protein components of urinary tract stone may provide a tool for monitoring the prognosis or relapse in the patients.",
author = "Kayo Yokomizoi and Aki Nakayama and Eisaku Hokazono and Akiko Ninomiya and Ruriko Miyake and Nobuo Hiratsuka and Mitsuhiko Okuyama and Yuji Kato and Shizuko Kobayashi and Yoshihisa Ito and Kiyoko Shiba",
year = "2005",
month = "1",
day = "1",
language = "English",
volume = "53",
pages = "1109--1115",
journal = "Rinsho byori. The Japanese journal of clinical pathology",
issn = "0047-1860",
publisher = "Nihon Rinsho Byori Gakkai",
number = "12",

}

TY - JOUR

T1 - Analysis of proteins in urinary tract stones and urine of urolithic patients

AU - Yokomizoi, Kayo

AU - Nakayama, Aki

AU - Hokazono, Eisaku

AU - Ninomiya, Akiko

AU - Miyake, Ruriko

AU - Hiratsuka, Nobuo

AU - Okuyama, Mitsuhiko

AU - Kato, Yuji

AU - Kobayashi, Shizuko

AU - Ito, Yoshihisa

AU - Shiba, Kiyoko

PY - 2005/1/1

Y1 - 2005/1/1

N2 - In order to investigate the mechanism of urinary tract stone formation, we analyzed protein components in urine and the stone. Urinary proteins of healthy subjects and urolithic patients as well as protein components urinary tract stone of the urolithic patients were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Electrophoretic patterns of urinary proteins of the patients differed from those of healthy subjects after separating protein patterns into those larger than 66kDa or smaller than 30kDa. Protein constituents of urinary tract stone were mainly separated into 18 bands ranging from 26.8 to 143 kDa. Major bands among these 18 bands differed among stones from different patients. On western blotting, the developed intensities of Tamm-Horsfall protein (THP) were fainter than those of healthy subjects. Whereas intensities of albumin (ALB) were stronger than those of healthy subjects. Moreover, blotting patterns of THP of the patients on non-reducing SDS-PAGE were obviously broad. Thus, we suggest that analysis of fractionated urinary proteins or protein components of urinary tract stone may provide a tool for monitoring the prognosis or relapse in the patients.

AB - In order to investigate the mechanism of urinary tract stone formation, we analyzed protein components in urine and the stone. Urinary proteins of healthy subjects and urolithic patients as well as protein components urinary tract stone of the urolithic patients were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Electrophoretic patterns of urinary proteins of the patients differed from those of healthy subjects after separating protein patterns into those larger than 66kDa or smaller than 30kDa. Protein constituents of urinary tract stone were mainly separated into 18 bands ranging from 26.8 to 143 kDa. Major bands among these 18 bands differed among stones from different patients. On western blotting, the developed intensities of Tamm-Horsfall protein (THP) were fainter than those of healthy subjects. Whereas intensities of albumin (ALB) were stronger than those of healthy subjects. Moreover, blotting patterns of THP of the patients on non-reducing SDS-PAGE were obviously broad. Thus, we suggest that analysis of fractionated urinary proteins or protein components of urinary tract stone may provide a tool for monitoring the prognosis or relapse in the patients.

UR - http://www.scopus.com/inward/record.url?scp=33644963390&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33644963390&partnerID=8YFLogxK

M3 - Article

C2 - 16447682

AN - SCOPUS:33644963390

VL - 53

SP - 1109

EP - 1115

JO - Rinsho byori. The Japanese journal of clinical pathology

JF - Rinsho byori. The Japanese journal of clinical pathology

SN - 0047-1860

IS - 12

ER -