Analysis of replicating mitochondrial DNA by two-dimensional agarose gel electrophoresis.

Aurelio Reyes, Takehiro Yasukawa, Ian J. Holt

Research output: Chapter in Book/Report/Conference proceedingChapter

10 Citations (Scopus)

Abstract

Replication intermediates can be separated on agarose gels in two dimensions to reveal a wealth of data on mechanisms of DNA replication. When applied to mitochondrial DNA of higher vertebrates, this technique unearthed a host of unexpected findings, the full implications of which are still being absorbed. Here, we describe the procedures we use to isolate intact mitochondrial replication intermediates from liver of higher vertebrates and the process of separating DNA fragments on neutral two-dimensional agarose gels.

Original languageEnglish
Title of host publicationMitochondria
Subtitle of host publicationPractical Protocols
Pages219-232
Number of pages14
Volume372
Publication statusPublished - 2007
Externally publishedYes

Publication series

NameMethods in Molecular Biology
PublisherHumana Press
ISSN (Print)1064-3745

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

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