ANALYSIS OF SECRETION AND EXPRESSION OF PLATELET‐DERIVED GROWTH FACTOR IN CULTURED ENDOTHELIAL CELLS FROM STROKE‐PRONE SPONTANEOUSLY HYPERTENSIVE RAT

Koji Iihara; Masakiyo Sasahara; Yukikazu Saeki; Nobuo Hashimoto; Haruhiko Kikuchi; Fumitada Hazama

doi:10.1111/j.1440-1681.1993.tb01734.x

ANALYSIS OF SECRETION AND EXPRESSION OF PLATELET‐DERIVED GROWTH FACTOR IN CULTURED ENDOTHELIAL CELLS FROM STROKE‐PRONE SPONTANEOUSLY HYPERTENSIVE RAT

Koji Iihara, Masakiyo Sasahara, Yukikazu Saeki, Nobuo Hashimoto, Haruhiko Kikuchi, Fumitada Hazama

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

1. We characterized the endothelial cell‐derived growth factors of SHRSP and Wistar Kyoto rats (WKY), respectively and found that platelet‐derived growth factor (PDGF)‐B chain related growth factor constituted a major portion of the mitogenic activity of the conditioned media of endothelial cells from both animals. There were no remarkable qualitative differences between the endothelial cell‐derived growth factors of SHRSP and WKY. 2. Northern analysis revealed that the expression of PDGF‐B chain was 2–4‐fold enhanced in cultured aortic endothelial cells of SHRSP. This enhanced expression of PDGF‐B chain, which may be induced under chronic hypertensive conditions, is suggested to contribute to the increase in endothelial cell‐derived growth factors reported in this animal.

Original language	English
Pages (from-to)	515-521
Number of pages	7
Journal	Clinical and Experimental Pharmacology and Physiology
Volume	20
Issue number	7-8
DOIs	https://doi.org/10.1111/j.1440-1681.1993.tb01734.x
Publication status	Published - 1993
Externally published	Yes

All Science Journal Classification (ASJC) codes

Physiology
Pharmacology
Physiology (medical)

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10.1111/j.1440-1681.1993.tb01734.x

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ANALYSIS OF SECRETION AND EXPRESSION OF PLATELET‐DERIVED GROWTH FACTOR IN CULTURED ENDOTHELIAL CELLS FROM STROKE‐PRONE SPONTANEOUSLY HYPERTENSIVE RAT. / Iihara, Koji; Sasahara, Masakiyo; Saeki, Yukikazu; Hashimoto, Nobuo; Kikuchi, Haruhiko; Hazama, Fumitada.

In: Clinical and Experimental Pharmacology and Physiology, Vol. 20, No. 7-8, 1993, p. 515-521.

Research output: Contribution to journal › Article › peer-review

Iihara, K, Sasahara, M, Saeki, Y, Hashimoto, N, Kikuchi, H & Hazama, F 1993, 'ANALYSIS OF SECRETION AND EXPRESSION OF PLATELET‐DERIVED GROWTH FACTOR IN CULTURED ENDOTHELIAL CELLS FROM STROKE‐PRONE SPONTANEOUSLY HYPERTENSIVE RAT', Clinical and Experimental Pharmacology and Physiology, vol. 20, no. 7-8, pp. 515-521. https://doi.org/10.1111/j.1440-1681.1993.tb01734.x

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abstract = "1. We characterized the endothelial cell‐derived growth factors of SHRSP and Wistar Kyoto rats (WKY), respectively and found that platelet‐derived growth factor (PDGF)‐B chain related growth factor constituted a major portion of the mitogenic activity of the conditioned media of endothelial cells from both animals. There were no remarkable qualitative differences between the endothelial cell‐derived growth factors of SHRSP and WKY. 2. Northern analysis revealed that the expression of PDGF‐B chain was 2–4‐fold enhanced in cultured aortic endothelial cells of SHRSP. This enhanced expression of PDGF‐B chain, which may be induced under chronic hypertensive conditions, is suggested to contribute to the increase in endothelial cell‐derived growth factors reported in this animal.",

author = "Koji Iihara and Masakiyo Sasahara and Yukikazu Saeki and Nobuo Hashimoto and Haruhiko Kikuchi and Fumitada Hazama",

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doi = "10.1111/j.1440-1681.1993.tb01734.x",

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AU - Iihara, Koji

AU - Sasahara, Masakiyo

AU - Saeki, Yukikazu

AU - Hashimoto, Nobuo

AU - Kikuchi, Haruhiko

AU - Hazama, Fumitada

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AB - 1. We characterized the endothelial cell‐derived growth factors of SHRSP and Wistar Kyoto rats (WKY), respectively and found that platelet‐derived growth factor (PDGF)‐B chain related growth factor constituted a major portion of the mitogenic activity of the conditioned media of endothelial cells from both animals. There were no remarkable qualitative differences between the endothelial cell‐derived growth factors of SHRSP and WKY. 2. Northern analysis revealed that the expression of PDGF‐B chain was 2–4‐fold enhanced in cultured aortic endothelial cells of SHRSP. This enhanced expression of PDGF‐B chain, which may be induced under chronic hypertensive conditions, is suggested to contribute to the increase in endothelial cell‐derived growth factors reported in this animal.

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ANALYSIS OF SECRETION AND EXPRESSION OF PLATELET‐DERIVED GROWTH FACTOR IN CULTURED ENDOTHELIAL CELLS FROM STROKE‐PRONE SPONTANEOUSLY HYPERTENSIVE RAT

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