Abstract
1. We characterized the endothelial cell‐derived growth factors of SHRSP and Wistar Kyoto rats (WKY), respectively and found that platelet‐derived growth factor (PDGF)‐B chain related growth factor constituted a major portion of the mitogenic activity of the conditioned media of endothelial cells from both animals. There were no remarkable qualitative differences between the endothelial cell‐derived growth factors of SHRSP and WKY. 2. Northern analysis revealed that the expression of PDGF‐B chain was 2–4‐fold enhanced in cultured aortic endothelial cells of SHRSP. This enhanced expression of PDGF‐B chain, which may be induced under chronic hypertensive conditions, is suggested to contribute to the increase in endothelial cell‐derived growth factors reported in this animal.
| Original language | English |
|---|---|
| Pages (from-to) | 515-521 |
| Number of pages | 7 |
| Journal | Clinical and Experimental Pharmacology and Physiology |
| Volume | 20 |
| Issue number | 7-8 |
| DOIs | |
| Publication status | Published - 1993 |
| Externally published | Yes |
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All Science Journal Classification (ASJC) codes
- Physiology
- Pharmacology
- Physiology (medical)
Cite this
ANALYSIS OF SECRETION AND EXPRESSION OF PLATELET‐DERIVED GROWTH FACTOR IN CULTURED ENDOTHELIAL CELLS FROM STROKE‐PRONE SPONTANEOUSLY HYPERTENSIVE RAT. / Iihara, Koji; Sasahara, Masakiyo; Saeki, Yukikazu; Hashimoto, Nobuo; Kikuchi, Haruhiko; Hazama, Fumitada.
In: Clinical and Experimental Pharmacology and Physiology, Vol. 20, No. 7-8, 1993, p. 515-521.Research output: Contribution to journal › Article
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TY - JOUR
T1 - ANALYSIS OF SECRETION AND EXPRESSION OF PLATELET‐DERIVED GROWTH FACTOR IN CULTURED ENDOTHELIAL CELLS FROM STROKE‐PRONE SPONTANEOUSLY HYPERTENSIVE RAT
AU - Iihara, Koji
AU - Sasahara, Masakiyo
AU - Saeki, Yukikazu
AU - Hashimoto, Nobuo
AU - Kikuchi, Haruhiko
AU - Hazama, Fumitada
PY - 1993
Y1 - 1993
N2 - 1. We characterized the endothelial cell‐derived growth factors of SHRSP and Wistar Kyoto rats (WKY), respectively and found that platelet‐derived growth factor (PDGF)‐B chain related growth factor constituted a major portion of the mitogenic activity of the conditioned media of endothelial cells from both animals. There were no remarkable qualitative differences between the endothelial cell‐derived growth factors of SHRSP and WKY. 2. Northern analysis revealed that the expression of PDGF‐B chain was 2–4‐fold enhanced in cultured aortic endothelial cells of SHRSP. This enhanced expression of PDGF‐B chain, which may be induced under chronic hypertensive conditions, is suggested to contribute to the increase in endothelial cell‐derived growth factors reported in this animal.
AB - 1. We characterized the endothelial cell‐derived growth factors of SHRSP and Wistar Kyoto rats (WKY), respectively and found that platelet‐derived growth factor (PDGF)‐B chain related growth factor constituted a major portion of the mitogenic activity of the conditioned media of endothelial cells from both animals. There were no remarkable qualitative differences between the endothelial cell‐derived growth factors of SHRSP and WKY. 2. Northern analysis revealed that the expression of PDGF‐B chain was 2–4‐fold enhanced in cultured aortic endothelial cells of SHRSP. This enhanced expression of PDGF‐B chain, which may be induced under chronic hypertensive conditions, is suggested to contribute to the increase in endothelial cell‐derived growth factors reported in this animal.
UR - http://www.scopus.com/inward/record.url?scp=0027237392&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0027237392&partnerID=8YFLogxK
U2 - 10.1111/j.1440-1681.1993.tb01734.x
DO - 10.1111/j.1440-1681.1993.tb01734.x
M3 - Article
VL - 20
SP - 515
EP - 521
JO - Clinical and Experimental Pharmacology and Physiology
JF - Clinical and Experimental Pharmacology and Physiology
SN - 0305-1870
IS - 7-8
ER -