TY - CHAP
T1 - Analysis of Small RNA Profiles During Development
AU - Watanabe, Toshiaki
AU - Totoki, Yasushi
AU - Sasaki, Hiroyuki
AU - Minami, Naojiro
AU - Imai, Hiroshi
PY - 2007
Y1 - 2007
N2 - Small RNAs ranging in size between 20 and 32 nt regulate gene expression through chromatin modification, mRNA degradation, and translational repression. Three major classes of small RNAs have been characterized: microRNAs (miRNAs), short interfering RNAs (siRNAs), and Piwi-interacting RNAs (piRNAs). miRNAs are expressed in a developmentally regulated and tissue-specific manner and are involved in development and cell differentiation. siRNAs are mainly involved in defense against transposons and viruses. piRNAs are expressed in germ cells and stem cells and are thought to repress transposition of retrotransposons. In this chapter, we describe the methods of small RNA cloning, annotation and classification, and their expression analysis during development.
AB - Small RNAs ranging in size between 20 and 32 nt regulate gene expression through chromatin modification, mRNA degradation, and translational repression. Three major classes of small RNAs have been characterized: microRNAs (miRNAs), short interfering RNAs (siRNAs), and Piwi-interacting RNAs (piRNAs). miRNAs are expressed in a developmentally regulated and tissue-specific manner and are involved in development and cell differentiation. siRNAs are mainly involved in defense against transposons and viruses. piRNAs are expressed in germ cells and stem cells and are thought to repress transposition of retrotransposons. In this chapter, we describe the methods of small RNA cloning, annotation and classification, and their expression analysis during development.
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U2 - 10.1016/S0076-6879(07)27009-0
DO - 10.1016/S0076-6879(07)27009-0
M3 - Chapter
C2 - 17720484
AN - SCOPUS:42949167336
SN - 9780123739179
T3 - Methods in Enzymology
SP - 155
EP - 169
BT - MicroRNA Methods
A2 - Rossi, John
A2 - Hannon, Gregory
ER -