Analysis of Small RNA Profiles During Development

Toshiaki Watanabe; Yasushi Totoki; Hiroyuki Sasaki; Naojiro Minami; Hiroshi Imai

doi:10.1016/S0076-6879(07)27009-0

Analysis of Small RNA Profiles During Development

Toshiaki Watanabe, Yasushi Totoki, Hiroyuki Sasaki, Naojiro Minami, Hiroshi Imai

Research output: Chapter in Book/Report/Conference proceeding › Chapter

10 Citations (Scopus)

Abstract

Small RNAs ranging in size between 20 and 32 nt regulate gene expression through chromatin modification, mRNA degradation, and translational repression. Three major classes of small RNAs have been characterized: microRNAs (miRNAs), short interfering RNAs (siRNAs), and Piwi-interacting RNAs (piRNAs). miRNAs are expressed in a developmentally regulated and tissue-specific manner and are involved in development and cell differentiation. siRNAs are mainly involved in defense against transposons and viruses. piRNAs are expressed in germ cells and stem cells and are thought to repress transposition of retrotransposons. In this chapter, we describe the methods of small RNA cloning, annotation and classification, and their expression analysis during development.

Original language	English
Title of host publication	MicroRNA Methods
Editors	John Rossi, Gregory Hannon
Pages	155-169
Number of pages	15
DOIs	https://doi.org/10.1016/S0076-6879(07)27009-0
Publication status	Published - Dec 1 2007
Externally published	Yes

Publication series

Name	Methods in Enzymology
Volume	427
ISSN (Print)	0076-6879

All Science Journal Classification (ASJC) codes

Biochemistry
Molecular Biology

Access to Document

10.1016/S0076-6879(07)27009-0

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Watanabe, T., Totoki, Y., Sasaki, H., Minami, N., & Imai, H. (2007). Analysis of Small RNA Profiles During Development. In J. Rossi, & G. Hannon (Eds.), MicroRNA Methods (pp. 155-169). (Methods in Enzymology; Vol. 427). https://doi.org/10.1016/S0076-6879(07)27009-0

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