Analysis of t cell response using motif aided peptide epitope liblary

Nobuhiro Kamikawaji, Tohru Sudo, Takehiko Sasazuki

Research output: Contribution to journalArticle

Abstract

We describe a novel method to investigate variability of T cell responses of PBLs using large libraries of synthetic pepiides. Motif aided peptide epitope library for DRB 1*0405 restricted T cell response was synthesized, containing fixed amino acids representing ihe major histocompatibility complex (MHC) class II anchor residues for HLA-DRB1*0405 (YXXLXAXXD). We synthesized 135 sets of peptide libraries, each of which contains 729 peptide species. T cell responses of PBLs were examined for two HLA-DR identical donors (DRB1*0405/1502) and one non identical donors (DRB 1*0406/0901). Appreciable T cell responses were observed in both donors. The response pattern of PBLs against two HLA-DR identical donors were similar while that between HLA-DR non identical donors were different, suggesting the strong HLA restriction of T cell response. The library will be useful tool to investigate the pathogenic T cell response such as auto immune diseases.

Original languageEnglish
Pages (from-to)102
Number of pages1
JournalJapanese Journal of Human Genetics
Volume41
Issue number1
Publication statusPublished - 1996

Fingerprint

Epitopes
T-Lymphocytes
Peptides
HLA-DR Antigens
Dichlororibofuranosylbenzimidazole
Peptide Library
Libraries
Immune System Diseases
Major Histocompatibility Complex
Amino Acids

All Science Journal Classification (ASJC) codes

  • Genetics(clinical)
  • Genetics

Cite this

Analysis of t cell response using motif aided peptide epitope liblary. / Kamikawaji, Nobuhiro; Sudo, Tohru; Sasazuki, Takehiko.

In: Japanese Journal of Human Genetics, Vol. 41, No. 1, 1996, p. 102.

Research output: Contribution to journalArticle

Kamikawaji, N, Sudo, T & Sasazuki, T 1996, 'Analysis of t cell response using motif aided peptide epitope liblary', Japanese Journal of Human Genetics, vol. 41, no. 1, pp. 102.
Kamikawaji, Nobuhiro ; Sudo, Tohru ; Sasazuki, Takehiko. / Analysis of t cell response using motif aided peptide epitope liblary. In: Japanese Journal of Human Genetics. 1996 ; Vol. 41, No. 1. pp. 102.
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