Analysis of the expression of cagA and vacA and the vacuolating activity in 167 isolates from patients with either peptic ulcers or non-ulcer dyspepsia

Tohru Takata, Shuji Fujimoto, Keizo Anzai, Takuro Shirotani, Mitsuo Okada, Yoshiro Sawae, Junko Ono

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Objectives: The goals of this study were: 1) to examine the prevalence of cytotoxin-associated protein (CagA), vacuolating cytotoxin (VacA), and the vacuolating cytotoxin activity (VCA) in vitro of infecting Helicobacter pylori isolates and 2) to clarify the relation between the expression of these virulence factors and the occurrence of peptic ulceration. Methods: One hundred sixty-seven clinical isolates of H. pylori from patients with peptic ulcer disease (gastric ulcer, 62 cases; duodenal ulcer, 48 cases) and nonulcer dyspepsia (57 cases) were studied regarding their genetic and phenotypic properties. Results: Type 1 bacteria, which had both CagA and VCA, and type 2 bacteria, which did not express either CagA or VCA, represented 62.9% and 7.8%, respectively; the remaining 29.4% had an intermediate phenotype, expressing either CagA independent of the presence of VCA (CagA+VCA-) or vice versa (CagA-VCA+). CagA+VCA- and CagA-VCA+ bacteria represented 17.4% and 12.0%, respectively, both of which were more numerous than the type 2 category. The proportion of the CagA-positive isolates was significantly higher in both the duodenal ulcer (97.9%) and gastric ulcer (83.9%) patients than in the non-ulcer dyspepsia patients (61.4%) (p < 0.01). On the other hand, the proportion of VacA/VCA-positive isolates was not significantly different between peptic ulcer disease and nonulcer dyspepsia. Conclusions: The currently used classification of this bacterium based on the concomitant expression of CagA and VacA/VCA into the two major types is not adequate. The CagA-positive phenotype thus may be important as a virulence marker for peptic ulcer disease independent of the presence of VacA/VCA.

Original languageEnglish
Pages (from-to)30-34
Number of pages5
JournalAmerican Journal of Gastroenterology
Volume93
Issue number1
DOIs
Publication statusPublished - Jan 1 1998

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Dyspepsia
Cytotoxins
Peptic Ulcer
Bacteria
Stomach Ulcer
Duodenal Ulcer
Helicobacter pylori
Phenotype
Virulence Factors
Virulence
Digestion

All Science Journal Classification (ASJC) codes

  • Hepatology
  • Gastroenterology

Cite this

Analysis of the expression of cagA and vacA and the vacuolating activity in 167 isolates from patients with either peptic ulcers or non-ulcer dyspepsia. / Takata, Tohru; Fujimoto, Shuji; Anzai, Keizo; Shirotani, Takuro; Okada, Mitsuo; Sawae, Yoshiro; Ono, Junko.

In: American Journal of Gastroenterology, Vol. 93, No. 1, 01.01.1998, p. 30-34.

Research output: Contribution to journalArticle

Takata, Tohru ; Fujimoto, Shuji ; Anzai, Keizo ; Shirotani, Takuro ; Okada, Mitsuo ; Sawae, Yoshiro ; Ono, Junko. / Analysis of the expression of cagA and vacA and the vacuolating activity in 167 isolates from patients with either peptic ulcers or non-ulcer dyspepsia. In: American Journal of Gastroenterology. 1998 ; Vol. 93, No. 1. pp. 30-34.
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abstract = "Objectives: The goals of this study were: 1) to examine the prevalence of cytotoxin-associated protein (CagA), vacuolating cytotoxin (VacA), and the vacuolating cytotoxin activity (VCA) in vitro of infecting Helicobacter pylori isolates and 2) to clarify the relation between the expression of these virulence factors and the occurrence of peptic ulceration. Methods: One hundred sixty-seven clinical isolates of H. pylori from patients with peptic ulcer disease (gastric ulcer, 62 cases; duodenal ulcer, 48 cases) and nonulcer dyspepsia (57 cases) were studied regarding their genetic and phenotypic properties. Results: Type 1 bacteria, which had both CagA and VCA, and type 2 bacteria, which did not express either CagA or VCA, represented 62.9{\%} and 7.8{\%}, respectively; the remaining 29.4{\%} had an intermediate phenotype, expressing either CagA independent of the presence of VCA (CagA+VCA-) or vice versa (CagA-VCA+). CagA+VCA- and CagA-VCA+ bacteria represented 17.4{\%} and 12.0{\%}, respectively, both of which were more numerous than the type 2 category. The proportion of the CagA-positive isolates was significantly higher in both the duodenal ulcer (97.9{\%}) and gastric ulcer (83.9{\%}) patients than in the non-ulcer dyspepsia patients (61.4{\%}) (p < 0.01). On the other hand, the proportion of VacA/VCA-positive isolates was not significantly different between peptic ulcer disease and nonulcer dyspepsia. Conclusions: The currently used classification of this bacterium based on the concomitant expression of CagA and VacA/VCA into the two major types is not adequate. The CagA-positive phenotype thus may be important as a virulence marker for peptic ulcer disease independent of the presence of VacA/VCA.",
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T1 - Analysis of the expression of cagA and vacA and the vacuolating activity in 167 isolates from patients with either peptic ulcers or non-ulcer dyspepsia

AU - Takata, Tohru

AU - Fujimoto, Shuji

AU - Anzai, Keizo

AU - Shirotani, Takuro

AU - Okada, Mitsuo

AU - Sawae, Yoshiro

AU - Ono, Junko

PY - 1998/1/1

Y1 - 1998/1/1

N2 - Objectives: The goals of this study were: 1) to examine the prevalence of cytotoxin-associated protein (CagA), vacuolating cytotoxin (VacA), and the vacuolating cytotoxin activity (VCA) in vitro of infecting Helicobacter pylori isolates and 2) to clarify the relation between the expression of these virulence factors and the occurrence of peptic ulceration. Methods: One hundred sixty-seven clinical isolates of H. pylori from patients with peptic ulcer disease (gastric ulcer, 62 cases; duodenal ulcer, 48 cases) and nonulcer dyspepsia (57 cases) were studied regarding their genetic and phenotypic properties. Results: Type 1 bacteria, which had both CagA and VCA, and type 2 bacteria, which did not express either CagA or VCA, represented 62.9% and 7.8%, respectively; the remaining 29.4% had an intermediate phenotype, expressing either CagA independent of the presence of VCA (CagA+VCA-) or vice versa (CagA-VCA+). CagA+VCA- and CagA-VCA+ bacteria represented 17.4% and 12.0%, respectively, both of which were more numerous than the type 2 category. The proportion of the CagA-positive isolates was significantly higher in both the duodenal ulcer (97.9%) and gastric ulcer (83.9%) patients than in the non-ulcer dyspepsia patients (61.4%) (p < 0.01). On the other hand, the proportion of VacA/VCA-positive isolates was not significantly different between peptic ulcer disease and nonulcer dyspepsia. Conclusions: The currently used classification of this bacterium based on the concomitant expression of CagA and VacA/VCA into the two major types is not adequate. The CagA-positive phenotype thus may be important as a virulence marker for peptic ulcer disease independent of the presence of VacA/VCA.

AB - Objectives: The goals of this study were: 1) to examine the prevalence of cytotoxin-associated protein (CagA), vacuolating cytotoxin (VacA), and the vacuolating cytotoxin activity (VCA) in vitro of infecting Helicobacter pylori isolates and 2) to clarify the relation between the expression of these virulence factors and the occurrence of peptic ulceration. Methods: One hundred sixty-seven clinical isolates of H. pylori from patients with peptic ulcer disease (gastric ulcer, 62 cases; duodenal ulcer, 48 cases) and nonulcer dyspepsia (57 cases) were studied regarding their genetic and phenotypic properties. Results: Type 1 bacteria, which had both CagA and VCA, and type 2 bacteria, which did not express either CagA or VCA, represented 62.9% and 7.8%, respectively; the remaining 29.4% had an intermediate phenotype, expressing either CagA independent of the presence of VCA (CagA+VCA-) or vice versa (CagA-VCA+). CagA+VCA- and CagA-VCA+ bacteria represented 17.4% and 12.0%, respectively, both of which were more numerous than the type 2 category. The proportion of the CagA-positive isolates was significantly higher in both the duodenal ulcer (97.9%) and gastric ulcer (83.9%) patients than in the non-ulcer dyspepsia patients (61.4%) (p < 0.01). On the other hand, the proportion of VacA/VCA-positive isolates was not significantly different between peptic ulcer disease and nonulcer dyspepsia. Conclusions: The currently used classification of this bacterium based on the concomitant expression of CagA and VacA/VCA into the two major types is not adequate. The CagA-positive phenotype thus may be important as a virulence marker for peptic ulcer disease independent of the presence of VacA/VCA.

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