Analysis of the human adrenodoxin promoter: Evidence for its activity

Bon chu Chung, Char Chang Lai, Cheng Hui Lin, Chi Yao Chang

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Adrenodoxin is an iron-sulfur protein serving as an electron transfer intermediate in the mitochondrial cytochrome P450 system. To study its transcriptional regulation we construct a human adrenodoxin genomic clone which includes 333 bp of DNA upstream of the mRNA start site. This DNA contains a TATA box and two GC boxes. When we place it in front of the CAT reporter gene and transfect it into the recipient cell lines, it directs transcription of the CAT gene. This DNA in reverse orientation does not show any transcriptional activity. This promoter element functions in three mammalian cell lines: JEG-3, COS-1, and Y-1.

Original languageEnglish
Pages (from-to)343-348
Number of pages6
JournalBiochemical and Biophysical Research Communications
Issue number1
Publication statusPublished - Feb 28 1989


All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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