TY - JOUR
T1 - Analysis of the human adrenodoxin promoter
T2 - Evidence for its activity
AU - Chung, Bon chu
AU - Lai, Char Chang
AU - Lin, Cheng Hui
AU - Chang, Chi Yao
N1 - Funding Information:
ACKNOWLEDGMENTS This work was supported by Academia Sinica and National Science Council, Republic of China. CYC is a predoctoral student at the Institute of Life Sciences, Tsing-Hwa University, Republic of China.
PY - 1989/2/28
Y1 - 1989/2/28
N2 - Adrenodoxin is an iron-sulfur protein serving as an electron transfer intermediate in the mitochondrial cytochrome P450 system. To study its transcriptional regulation we construct a human adrenodoxin genomic clone which includes 333 bp of DNA upstream of the mRNA start site. This DNA contains a TATA box and two GC boxes. When we place it in front of the CAT reporter gene and transfect it into the recipient cell lines, it directs transcription of the CAT gene. This DNA in reverse orientation does not show any transcriptional activity. This promoter element functions in three mammalian cell lines: JEG-3, COS-1, and Y-1.
AB - Adrenodoxin is an iron-sulfur protein serving as an electron transfer intermediate in the mitochondrial cytochrome P450 system. To study its transcriptional regulation we construct a human adrenodoxin genomic clone which includes 333 bp of DNA upstream of the mRNA start site. This DNA contains a TATA box and two GC boxes. When we place it in front of the CAT reporter gene and transfect it into the recipient cell lines, it directs transcription of the CAT gene. This DNA in reverse orientation does not show any transcriptional activity. This promoter element functions in three mammalian cell lines: JEG-3, COS-1, and Y-1.
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U2 - 10.1016/0006-291X(89)92444-3
DO - 10.1016/0006-291X(89)92444-3
M3 - Article
C2 - 2784316
AN - SCOPUS:0024593125
VL - 159
SP - 343
EP - 348
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 1
ER -