Analysis of the human adrenodoxin promoter: Evidence for its activity

Bon chu Chung; Char Chang Lai; Cheng Hui Lin; Chi Yao Chang

doi:10.1016/0006-291X(89)92444-3

Analysis of the human adrenodoxin promoter: Evidence for its activity

Bon chu Chung, Char Chang Lai, Cheng Hui Lin, Chi Yao Chang

Faculty of Medical Sciences

Research output: Contribution to journal › Article

8 Citations (Scopus)

Abstract

Adrenodoxin is an iron-sulfur protein serving as an electron transfer intermediate in the mitochondrial cytochrome P450 system. To study its transcriptional regulation we construct a human adrenodoxin genomic clone which includes 333 bp of DNA upstream of the mRNA start site. This DNA contains a TATA box and two GC boxes. When we place it in front of the CAT reporter gene and transfect it into the recipient cell lines, it directs transcription of the CAT gene. This DNA in reverse orientation does not show any transcriptional activity. This promoter element functions in three mammalian cell lines: JEG-3, COS-1, and Y-1.

Original language	English
Pages (from-to)	343-348
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	159
Issue number	1
DOIs	https://doi.org/10.1016/0006-291X(89)92444-3
Publication status	Published - Feb 28 1989

Fingerprint

All Science Journal Classification (ASJC) codes

Biophysics
Biochemistry
Molecular Biology
Cell Biology

Cite this

Chung, B. C., Lai, C. C., Lin, C. H., & Chang, C. Y. (1989). Analysis of the human adrenodoxin promoter: Evidence for its activity. Biochemical and Biophysical Research Communications, 159(1), 343-348. https://doi.org/10.1016/0006-291X(89)92444-3

@article{ab622efad439471eada7a0ccbeb69fa1,

title = "Analysis of the human adrenodoxin promoter: Evidence for its activity",

abstract = "Adrenodoxin is an iron-sulfur protein serving as an electron transfer intermediate in the mitochondrial cytochrome P450 system. To study its transcriptional regulation we construct a human adrenodoxin genomic clone which includes 333 bp of DNA upstream of the mRNA start site. This DNA contains a TATA box and two GC boxes. When we place it in front of the CAT reporter gene and transfect it into the recipient cell lines, it directs transcription of the CAT gene. This DNA in reverse orientation does not show any transcriptional activity. This promoter element functions in three mammalian cell lines: JEG-3, COS-1, and Y-1.",

author = "Chung, {Bon chu} and Lai, {Char Chang} and Lin, {Cheng Hui} and Chang, {Chi Yao}",

year = "1989",

month = "2",

day = "28",

doi = "10.1016/0006-291X(89)92444-3",

language = "English",

volume = "159",

pages = "343--348",

journal = "Biochemical and Biophysical Research Communications",

issn = "0006-291X",

publisher = "Academic Press Inc.",

number = "1",

}

TY - JOUR

T1 - Analysis of the human adrenodoxin promoter

T2 - Evidence for its activity

AU - Chung, Bon chu

AU - Lai, Char Chang

AU - Lin, Cheng Hui

AU - Chang, Chi Yao

PY - 1989/2/28

Y1 - 1989/2/28

N2 - Adrenodoxin is an iron-sulfur protein serving as an electron transfer intermediate in the mitochondrial cytochrome P450 system. To study its transcriptional regulation we construct a human adrenodoxin genomic clone which includes 333 bp of DNA upstream of the mRNA start site. This DNA contains a TATA box and two GC boxes. When we place it in front of the CAT reporter gene and transfect it into the recipient cell lines, it directs transcription of the CAT gene. This DNA in reverse orientation does not show any transcriptional activity. This promoter element functions in three mammalian cell lines: JEG-3, COS-1, and Y-1.

AB - Adrenodoxin is an iron-sulfur protein serving as an electron transfer intermediate in the mitochondrial cytochrome P450 system. To study its transcriptional regulation we construct a human adrenodoxin genomic clone which includes 333 bp of DNA upstream of the mRNA start site. This DNA contains a TATA box and two GC boxes. When we place it in front of the CAT reporter gene and transfect it into the recipient cell lines, it directs transcription of the CAT gene. This DNA in reverse orientation does not show any transcriptional activity. This promoter element functions in three mammalian cell lines: JEG-3, COS-1, and Y-1.

UR - http://www.scopus.com/inward/record.url?scp=0024593125&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024593125&partnerID=8YFLogxK

U2 - 10.1016/0006-291X(89)92444-3

DO - 10.1016/0006-291X(89)92444-3

M3 - Article

C2 - 2784316

AN - SCOPUS:0024593125

VL - 159

SP - 343

EP - 348

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 1

ER -

Access to Document

10.1016/0006-291X(89)92444-3