TY - JOUR
T1 - Analysis of the Molecular Recognition of L-lsoleucine by Isoleucyl-tRNA Synthetase
AU - Yokoyama, Shigeyuki
AU - Kohda, Daisuke
AU - Miyazawa, Tatsuo
PY - 1987
Y1 - 1987
N2 - The 400-MHz 1H-NMR spectra of L-isoleucine were measured in the presence of Escherichia coli isoleucyl-tRNA synthetase (IleRS). Because of chemical exchange of L-isoleucine between the free state and the IleRS-bound state, transferred nuclear Overhauser effect (TRNOE) was observed among proton resonances of L-isoleucine. The IleRS-bound L-isoleucine was found to take the gauche+ form about the Cα-Cβ bond and the trans form about the Cβ-Cγ1 bond. The TRNOE analysis is useful for studying the amino acid discrimination mechanism of ammoacyl-tRNA synthetases.
AB - The 400-MHz 1H-NMR spectra of L-isoleucine were measured in the presence of Escherichia coli isoleucyl-tRNA synthetase (IleRS). Because of chemical exchange of L-isoleucine between the free state and the IleRS-bound state, transferred nuclear Overhauser effect (TRNOE) was observed among proton resonances of L-isoleucine. The IleRS-bound L-isoleucine was found to take the gauche+ form about the Cα-Cβ bond and the trans form about the Cβ-Cγ1 bond. The TRNOE analysis is useful for studying the amino acid discrimination mechanism of ammoacyl-tRNA synthetases.
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U2 - 10.1246/nikkashi.1987.402
DO - 10.1246/nikkashi.1987.402
M3 - Article
AN - SCOPUS:85016522098
SN - 0369-4577
VL - 1987
SP - 402
EP - 404
JO - Nippon Kagaku Kaishi / Chemical Society of Japan - Chemistry and Industrial Chemistry Journal
JF - Nippon Kagaku Kaishi / Chemical Society of Japan - Chemistry and Industrial Chemistry Journal
IS - 3
ER -