Analysis of the promoter of mutated human whey acidic protein (WAP) gene.

Naoko Nukumi, Mami Seki, Tokuko Iwamori, Tetsushi Yada, Kunihiko Naito, Hideaki Tojo

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Although whey acidic protein (WAP) has been identified in the milk of a range of species, it has been predicted that WAP is not secreted into human milk as a result of critical point mutations within the coding region. In the present study, we first investigated computationally the promoter region of mutated human WAP genes by comparing with those of other known WAP genes. Computational database analyses showed that the human WAP promoter region was highly conserved, as in other species with milk WAP. Next, we evaluated the activity of the human WAP promoter (2.6 kb) using a reporter gene assay. MCF-7 cells were stably transfected with the hWAP/hGH (human growth hormone) fusion gene, cultured on Matrigel, and treated with lactogenic hormones. Radioimmunoassay detected hGH in the culture medium, indicating that the human WAP promoter was responsible for the lactogenic hormones. The human WAP promoter was significantly more active in MCF-7 cells than the mouse WAP promoter (2.4 kb). The present results provide us with important information on the molecular evolution of milk protein genes.

Original languageEnglish
Pages (from-to)315-320
Number of pages6
JournalThe Journal of reproduction and development
Volume52
Issue number2
DOIs
Publication statusPublished - Jan 1 2006
Externally publishedYes

Fingerprint

whey
promoter regions
genes
proteins
prolactin
milk
human growth
gene fusion
point mutation
breast milk
radioimmunoassays
dairy protein
reporter genes
somatotropin
culture media
cells
mice
assays

All Science Journal Classification (ASJC) codes

  • Animal Science and Zoology

Cite this

Analysis of the promoter of mutated human whey acidic protein (WAP) gene. / Nukumi, Naoko; Seki, Mami; Iwamori, Tokuko; Yada, Tetsushi; Naito, Kunihiko; Tojo, Hideaki.

In: The Journal of reproduction and development, Vol. 52, No. 2, 01.01.2006, p. 315-320.

Research output: Contribution to journalArticle

Nukumi, Naoko ; Seki, Mami ; Iwamori, Tokuko ; Yada, Tetsushi ; Naito, Kunihiko ; Tojo, Hideaki. / Analysis of the promoter of mutated human whey acidic protein (WAP) gene. In: The Journal of reproduction and development. 2006 ; Vol. 52, No. 2. pp. 315-320.
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