Analysis of the Squamous Cell Carcinoma Antigen (SCCA) Which Has a Significant Difference in Values between CLIA and FEIA--Case Report of Extra Glycosylation in SCCA

Kanae Maruyama; Yoshimasa Aoki; Motoko Yamanaka; Miyuki Ono; Taeko Hotta; Dongchon Kang

Analysis of the Squamous Cell Carcinoma Antigen (SCCA) Which Has a Significant Difference in Values between CLIA and FEIA--Case Report of Extra Glycosylation in SCCA

Kanae Maruyama, Yoshimasa Aoki, Motoko Yamanaka, Miyuki Ono, Taeko Hotta, Dongchon Kang

Department of Basic Medicine

Research output: Contribution to journal › Article › peer-review

Abstract

Squamous cell carcinoma antigen (SCCA) is a glycoprotein that belongs to the serine protease inhibitor family. Clinically, it has been utilized as a tumor marker for squamous cell carcinoma. In clinical laboratories, SCCA is measured by several immunoassays. Recently, a number of studies have been reported that there is a significant difference in values between the immunoassays, attributing to SCCA-immunoglobulin complex. We found a case with significant difference in the SCCA value between CLIA and FEIA. In this case, SCCA-Immunoglobulin complex was not confirmed by gel filtration analysis. Interestingly, 5 to 10 kDa slightly-high molecular weight SCCA compared to control was detected by immunoblotting assay. It may be suspected that the aberrant glycosyl modification of SCCA influenced the reactivity to immunoassays.

Original language	English
Pages (from-to)	265-269
Number of pages	5
Journal	Rinsho byori. The Japanese journal of clinical pathology
Volume	64
Issue number	3
Publication status	Published - Mar 1 2016

All Science Journal Classification (ASJC) codes

Medicine(all)

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Analysis of the Squamous Cell Carcinoma Antigen (SCCA) Which Has a Significant Difference in Values between CLIA and FEIA--Case Report of Extra Glycosylation in SCCA. / Maruyama, Kanae; Aoki, Yoshimasa; Yamanaka, Motoko; Ono, Miyuki; Hotta, Taeko; Kang, Dongchon.

In: Rinsho byori. The Japanese journal of clinical pathology, Vol. 64, No. 3, 01.03.2016, p. 265-269.

Research output: Contribution to journal › Article › peer-review

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abstract = "Squamous cell carcinoma antigen (SCCA) is a glycoprotein that belongs to the serine protease inhibitor family. Clinically, it has been utilized as a tumor marker for squamous cell carcinoma. In clinical laboratories, SCCA is measured by several immunoassays. Recently, a number of studies have been reported that there is a significant difference in values between the immunoassays, attributing to SCCA-immunoglobulin complex. We found a case with significant difference in the SCCA value between CLIA and FEIA. In this case, SCCA-Immunoglobulin complex was not confirmed by gel filtration analysis. Interestingly, 5 to 10 kDa slightly-high molecular weight SCCA compared to control was detected by immunoblotting assay. It may be suspected that the aberrant glycosyl modification of SCCA influenced the reactivity to immunoassays.",

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