Enzymes have been widely discussed to utilize various modes of molecular motions for their function. We have reported that enzyme activities can be controlled by ultrasound irradiation depending on its frequency, and the weak ultrasound can induce conformational changes of enzyme. In this study, we applied this methodology to DNA polymerase reaction on a DNA-immobilized 27 MHz QCM. We will discuss the ultrasound effects on the binding, polymerization and dissociation processes of DNA polymerase by means of frequency change (mass change) of QCM.
|Number of pages||1|
|Publication status||Published - Dec 1 2005|
|Event||54th SPSJ Annual Meeting 2005 - Yokohama, Japan|
Duration: May 25 2005 → May 27 2005
|Other||54th SPSJ Annual Meeting 2005|
|Period||5/25/05 → 5/27/05|
All Science Journal Classification (ASJC) codes