Analysis of weak ultrasound effect on DMA polymerase activity using a quartz crystal microbalance(QCM)

Yu Hoshino, Takayoshi Kawasaki, Yoshio Okahata

Research output: Contribution to conferencePaper

Abstract

Enzymes have been widely discussed to utilize various modes of molecular motions for their function. We have reported that enzyme activities can be controlled by ultrasound irradiation depending on its frequency, and the weak ultrasound can induce conformational changes of enzyme. In this study, we applied this methodology to DNA polymerase reaction on a DNA-immobilized 27 MHz QCM. We will discuss the ultrasound effects on the binding, polymerization and dissociation processes of DNA polymerase by means of frequency change (mass change) of QCM.

Original languageEnglish
Number of pages1
Publication statusPublished - Dec 1 2005
Externally publishedYes
Event54th SPSJ Annual Meeting 2005 - Yokohama, Japan
Duration: May 25 2005May 27 2005

Other

Other54th SPSJ Annual Meeting 2005
CountryJapan
CityYokohama
Period5/25/055/27/05

Fingerprint

Quartz crystal microbalances
Dynamic mechanical analysis
DNA
Ultrasonics
Enzymes
Enzyme activity
Polymerization
Irradiation

All Science Journal Classification (ASJC) codes

  • Engineering(all)

Cite this

Hoshino, Y., Kawasaki, T., & Okahata, Y. (2005). Analysis of weak ultrasound effect on DMA polymerase activity using a quartz crystal microbalance(QCM). Paper presented at 54th SPSJ Annual Meeting 2005, Yokohama, Japan.

Analysis of weak ultrasound effect on DMA polymerase activity using a quartz crystal microbalance(QCM). / Hoshino, Yu; Kawasaki, Takayoshi; Okahata, Yoshio.

2005. Paper presented at 54th SPSJ Annual Meeting 2005, Yokohama, Japan.

Research output: Contribution to conferencePaper

Hoshino, Y, Kawasaki, T & Okahata, Y 2005, 'Analysis of weak ultrasound effect on DMA polymerase activity using a quartz crystal microbalance(QCM)', Paper presented at 54th SPSJ Annual Meeting 2005, Yokohama, Japan, 5/25/05 - 5/27/05.
Hoshino Y, Kawasaki T, Okahata Y. Analysis of weak ultrasound effect on DMA polymerase activity using a quartz crystal microbalance(QCM). 2005. Paper presented at 54th SPSJ Annual Meeting 2005, Yokohama, Japan.
Hoshino, Yu ; Kawasaki, Takayoshi ; Okahata, Yoshio. / Analysis of weak ultrasound effect on DMA polymerase activity using a quartz crystal microbalance(QCM). Paper presented at 54th SPSJ Annual Meeting 2005, Yokohama, Japan.1 p.
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