Analysis of zebrafish cyp19 promoters

Sok Keng Tong, Bon Chu Chung

Research output: Contribution to journalArticle

80 Citations (Scopus)

Abstract

Cyp19 encodes P450 aromatase, the key enzyme catalyzing the conversion of androgens into estrogens. Estrogens play a crucial role in the anatomical, functional and behavioral characteristics of sexually dimorphic development. In zebrafish, two cyp19 genes, cyp19a and cyp19b, expressed in ovary and brain, respectively, were found. We have isolated the promoter regions of the zebrafish cyp19 genes from a bacterial artificial chromosome library to search for regulatory sequences that bind to transcription factors. Sequences like arylhydrocarbon receptor (AhR) recognition site, estrogen receptor recognition half sites (1/2ERE) and c-AMP responsive elements were found in the 5′-flanking regions of both cyp19 genes. For ovarian-specific expression, we found binding sites for steroidogenic factor-1 (SF-1), GATA transcription factor 4 (GATA-4) and Wilm tumor 1 (WT1-KTS) on the promoter region of cyp19a but not cyp19b. For brain-specific expression of the cyp19b gene, sequences for recognition of chicken ovalbumin upstream promoter-transcription factor (COUP) and Ptx-1 were detected in the promoter. The importance of these putative control elements in ovary and brain-specific promoter has been assessed by sequence comparison among various species.

Original languageEnglish
Pages (from-to)381-386
Number of pages6
JournalJournal of Steroid Biochemistry and Molecular Biology
Volume86
Issue number3-5
DOIs
Publication statusPublished - Sep 2003

Fingerprint

Zebrafish
Genes
Genetic Promoter Regions
Brain
Ovary
COUP Transcription Factors
GATA4 Transcription Factor
Estrogens
Steroidogenic Factor 1
Bacterial Artificial Chromosomes
Aromatase
5' Flanking Region
Adenosine Monophosphate
Estrogen Receptors
Androgens
Transcription Factors
Chromosomes
Binding Sites
Gene Expression
Tumors

All Science Journal Classification (ASJC) codes

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Endocrinology
  • Clinical Biochemistry
  • Cell Biology

Cite this

Analysis of zebrafish cyp19 promoters. / Tong, Sok Keng; Chung, Bon Chu.

In: Journal of Steroid Biochemistry and Molecular Biology, Vol. 86, No. 3-5, 09.2003, p. 381-386.

Research output: Contribution to journalArticle

Tong, Sok Keng ; Chung, Bon Chu. / Analysis of zebrafish cyp19 promoters. In: Journal of Steroid Biochemistry and Molecular Biology. 2003 ; Vol. 86, No. 3-5. pp. 381-386.
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