Analytical evaluation of bioactive small peptides on their intestinal absorption and bioavailability

Research output: Contribution to journalArticle

Abstract

Small peptides with di- or tripeptide length have been known as bioactive food components to prevent lifestyle-related diseases (e.g. hypertenstion). Although they must be absorbed into the blood circulation or accumulated in targeted tissues after their intake, the bioavailability was still uncertain due to limitations of analytical methods. Thus, the aim of this study was to develop analytical methods to measure these small peptides based on mass spectrometry (MS) techniques. In this study, a 2,4,6-trinitrobenzenesulfonate-derivatized liquid chromatography- multiple reaction monitoring-tandem mass spectrometry (LC-MRM-MS/MS) technique was developed to significantly enhance the sensitivity of small amines including peptides. The evidence of phytic acid to improve poor detection of small peptides (< 500 Da) by matrixassisted laser desorption/ionization-imaging mass spectrometry (MALDI-IMS) was also demonstrated. Here, this review article summarizes these developed MS techniques as well as the resulting bioabailability and intestinal absorption behavior of these bioactive small peptides based on the established analytical methods.

Original languageJapanese
Pages (from-to)677-685
Number of pages9
JournalBunseki Kagaku
Volume66
Issue number9
DOIs
Publication statusPublished - Jan 1 2017

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Mass spectrometry
Peptides
Phytic Acid
Dipeptides
Liquid chromatography
Hemodynamics
Ionization
Amines
Biological Availability
Desorption
Tissue
Imaging techniques
Lasers
Monitoring
Chemical analysis

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

Cite this

Analytical evaluation of bioactive small peptides on their intestinal absorption and bioavailability. / Tanaka, Mitsuru.

In: Bunseki Kagaku, Vol. 66, No. 9, 01.01.2017, p. 677-685.

Research output: Contribution to journalArticle

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