Anchored ISSR as molecular marker to characterize accessions of jacaranda mimosifolia L. Don.

A. S. Escandón, M. Pérez De La Torre, A. Acevedo, S. Marcucci-Poltri, Ikuo Miyajima

Research output: Chapter in Book/Report/Conference proceedingConference contribution

6 Citations (Scopus)

Abstract

Anchored inter-simple sequence repeats (ISSR) were used to identify 21 accessions of Jacaranda mimosifolia, collected mainly in Buenos Aires City, and the others in Mendoza, Salta and Jujuy Province and La Plata City, as part of an ornamental breeding program. DNA was extracted and purified from young leaves and amplified by four anchored primers based on dinucleotide repeats with 5′ or 3′extension. A total of 42 polymorphic bands, obtained after electrophoresis in agarose gels stained with ethidium bromide, were used to construct a molecular identification matrix. A mean of 12.75 bands per primer were generated, ranging from 140 to 1,400 bp. The genomic relationship of the genotypes was evaluated using Jaccard coefficient, and the dendrogram was constructed using UPGMA linkage method. All the accessions were differentiated by this technique, which showed a range of Resolving Power from 1.2 to 5.721. The anchored microsatellite analysis was shown to be a promising method to identify selected material in a breeding program of J. mimosifolia and with high potential to be applied to other ornamental crops.

Original languageEnglish
Title of host publicationV International Symposium on New Floricultural Crops
EditorsA.F.C. Tombolato, G.M. Dias-Tagliacozzo
Publication statusPublished - Dec 1 2005
Externally publishedYes

Publication series

NameActa Horticulturae
Volume683
ISSN (Print)0567-7572

Fingerprint

Jacaranda mimosifolia
microsatellite repeats
genetic markers
breeding
ornamental plants
agarose
electrophoresis
linkage (genetics)
methodology
gels
genomics
genotype
DNA
leaves

All Science Journal Classification (ASJC) codes

  • Horticulture

Cite this

Escandón, A. S., Pérez De La Torre, M., Acevedo, A., Marcucci-Poltri, S., & Miyajima, I. (2005). Anchored ISSR as molecular marker to characterize accessions of jacaranda mimosifolia L. Don. In A. F. C. Tombolato, & G. M. Dias-Tagliacozzo (Eds.), V International Symposium on New Floricultural Crops (Acta Horticulturae; Vol. 683).

Anchored ISSR as molecular marker to characterize accessions of jacaranda mimosifolia L. Don. / Escandón, A. S.; Pérez De La Torre, M.; Acevedo, A.; Marcucci-Poltri, S.; Miyajima, Ikuo.

V International Symposium on New Floricultural Crops. ed. / A.F.C. Tombolato; G.M. Dias-Tagliacozzo. 2005. (Acta Horticulturae; Vol. 683).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Escandón, AS, Pérez De La Torre, M, Acevedo, A, Marcucci-Poltri, S & Miyajima, I 2005, Anchored ISSR as molecular marker to characterize accessions of jacaranda mimosifolia L. Don. in AFC Tombolato & GM Dias-Tagliacozzo (eds), V International Symposium on New Floricultural Crops. Acta Horticulturae, vol. 683.
Escandón AS, Pérez De La Torre M, Acevedo A, Marcucci-Poltri S, Miyajima I. Anchored ISSR as molecular marker to characterize accessions of jacaranda mimosifolia L. Don. In Tombolato AFC, Dias-Tagliacozzo GM, editors, V International Symposium on New Floricultural Crops. 2005. (Acta Horticulturae).
Escandón, A. S. ; Pérez De La Torre, M. ; Acevedo, A. ; Marcucci-Poltri, S. ; Miyajima, Ikuo. / Anchored ISSR as molecular marker to characterize accessions of jacaranda mimosifolia L. Don. V International Symposium on New Floricultural Crops. editor / A.F.C. Tombolato ; G.M. Dias-Tagliacozzo. 2005. (Acta Horticulturae).
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