Androgen regulates Mafb expression through its 3′UTR during mouse urethral masculinization

Shoko Matsushita, Kentaro Suzuki, Yukiko Ogino, Shinjiro Hino, Tetsuya Sato, Mikita Suyama, Takahiro Matsumoto, Akiko Omori, Satoshi Inoue, Gen Yamada

    Research output: Contribution to journalArticle

    6 Citations (Scopus)

    Abstract

    External genitalia are prominent organs showing hormone-dependent sexual differentiation. Androgen is an essential regulator of masculinization of the genital tubercle, which is the anlage of external genitalia. We have previously shown that v-maf avian musculoaponeurotic fibrosarcoma oncogene homolog B (MAFB) is an androgen-inducible regulator of embryonic urethral masculinization in mice. However, it remains unclear how androgen regulates Mafb expression. The current study suggests that the Mafb 3′ untranslated region (UTR) is an essential region for its regulation by androgen. We identified 2 functional androgen response elements (AREs) in Mafb 3′UTR. Androgen receptor is bound to such AREs in 3′UTR during urethral masculinization. In addition to 3′UTR, Mafb 5′UTR also showed androgen responsiveness. Moreover, we also demonstrated that β-catenin, one of genital tubercle masculinization factors, may be an additional regulator of Mafb expression during urethral masculinization. This study provides insights to elucidate mechanisms of gene regulation through AREs present in Mafb 3′UTR for a better understanding of the processes of urethral masculinization.

    Original languageEnglish
    Pages (from-to)844-857
    Number of pages14
    JournalEndocrinology
    Volume157
    Issue number2
    DOIs
    Publication statusPublished - Feb 1 2016

    Fingerprint

    Androgens
    Response Elements
    Genitalia
    Catenins
    Sex Differentiation
    Fibrosarcoma
    Androgen Receptors
    3' Untranslated Regions
    Oncogenes
    Hormones
    Genes

    All Science Journal Classification (ASJC) codes

    • Endocrinology

    Cite this

    Androgen regulates Mafb expression through its 3′UTR during mouse urethral masculinization. / Matsushita, Shoko; Suzuki, Kentaro; Ogino, Yukiko; Hino, Shinjiro; Sato, Tetsuya; Suyama, Mikita; Matsumoto, Takahiro; Omori, Akiko; Inoue, Satoshi; Yamada, Gen.

    In: Endocrinology, Vol. 157, No. 2, 01.02.2016, p. 844-857.

    Research output: Contribution to journalArticle

    Matsushita, S, Suzuki, K, Ogino, Y, Hino, S, Sato, T, Suyama, M, Matsumoto, T, Omori, A, Inoue, S & Yamada, G 2016, 'Androgen regulates Mafb expression through its 3′UTR during mouse urethral masculinization', Endocrinology, vol. 157, no. 2, pp. 844-857. https://doi.org/10.1210/en.2015-1586
    Matsushita, Shoko ; Suzuki, Kentaro ; Ogino, Yukiko ; Hino, Shinjiro ; Sato, Tetsuya ; Suyama, Mikita ; Matsumoto, Takahiro ; Omori, Akiko ; Inoue, Satoshi ; Yamada, Gen. / Androgen regulates Mafb expression through its 3′UTR during mouse urethral masculinization. In: Endocrinology. 2016 ; Vol. 157, No. 2. pp. 844-857.
    @article{18c0ee34c4ca42d4ad98f62e84dc7324,
    title = "Androgen regulates Mafb expression through its 3′UTR during mouse urethral masculinization",
    abstract = "External genitalia are prominent organs showing hormone-dependent sexual differentiation. Androgen is an essential regulator of masculinization of the genital tubercle, which is the anlage of external genitalia. We have previously shown that v-maf avian musculoaponeurotic fibrosarcoma oncogene homolog B (MAFB) is an androgen-inducible regulator of embryonic urethral masculinization in mice. However, it remains unclear how androgen regulates Mafb expression. The current study suggests that the Mafb 3′ untranslated region (UTR) is an essential region for its regulation by androgen. We identified 2 functional androgen response elements (AREs) in Mafb 3′UTR. Androgen receptor is bound to such AREs in 3′UTR during urethral masculinization. In addition to 3′UTR, Mafb 5′UTR also showed androgen responsiveness. Moreover, we also demonstrated that β-catenin, one of genital tubercle masculinization factors, may be an additional regulator of Mafb expression during urethral masculinization. This study provides insights to elucidate mechanisms of gene regulation through AREs present in Mafb 3′UTR for a better understanding of the processes of urethral masculinization.",
    author = "Shoko Matsushita and Kentaro Suzuki and Yukiko Ogino and Shinjiro Hino and Tetsuya Sato and Mikita Suyama and Takahiro Matsumoto and Akiko Omori and Satoshi Inoue and Gen Yamada",
    year = "2016",
    month = "2",
    day = "1",
    doi = "10.1210/en.2015-1586",
    language = "English",
    volume = "157",
    pages = "844--857",
    journal = "Endocrinology",
    issn = "0013-7227",
    publisher = "The Endocrine Society",
    number = "2",

    }

    TY - JOUR

    T1 - Androgen regulates Mafb expression through its 3′UTR during mouse urethral masculinization

    AU - Matsushita, Shoko

    AU - Suzuki, Kentaro

    AU - Ogino, Yukiko

    AU - Hino, Shinjiro

    AU - Sato, Tetsuya

    AU - Suyama, Mikita

    AU - Matsumoto, Takahiro

    AU - Omori, Akiko

    AU - Inoue, Satoshi

    AU - Yamada, Gen

    PY - 2016/2/1

    Y1 - 2016/2/1

    N2 - External genitalia are prominent organs showing hormone-dependent sexual differentiation. Androgen is an essential regulator of masculinization of the genital tubercle, which is the anlage of external genitalia. We have previously shown that v-maf avian musculoaponeurotic fibrosarcoma oncogene homolog B (MAFB) is an androgen-inducible regulator of embryonic urethral masculinization in mice. However, it remains unclear how androgen regulates Mafb expression. The current study suggests that the Mafb 3′ untranslated region (UTR) is an essential region for its regulation by androgen. We identified 2 functional androgen response elements (AREs) in Mafb 3′UTR. Androgen receptor is bound to such AREs in 3′UTR during urethral masculinization. In addition to 3′UTR, Mafb 5′UTR also showed androgen responsiveness. Moreover, we also demonstrated that β-catenin, one of genital tubercle masculinization factors, may be an additional regulator of Mafb expression during urethral masculinization. This study provides insights to elucidate mechanisms of gene regulation through AREs present in Mafb 3′UTR for a better understanding of the processes of urethral masculinization.

    AB - External genitalia are prominent organs showing hormone-dependent sexual differentiation. Androgen is an essential regulator of masculinization of the genital tubercle, which is the anlage of external genitalia. We have previously shown that v-maf avian musculoaponeurotic fibrosarcoma oncogene homolog B (MAFB) is an androgen-inducible regulator of embryonic urethral masculinization in mice. However, it remains unclear how androgen regulates Mafb expression. The current study suggests that the Mafb 3′ untranslated region (UTR) is an essential region for its regulation by androgen. We identified 2 functional androgen response elements (AREs) in Mafb 3′UTR. Androgen receptor is bound to such AREs in 3′UTR during urethral masculinization. In addition to 3′UTR, Mafb 5′UTR also showed androgen responsiveness. Moreover, we also demonstrated that β-catenin, one of genital tubercle masculinization factors, may be an additional regulator of Mafb expression during urethral masculinization. This study provides insights to elucidate mechanisms of gene regulation through AREs present in Mafb 3′UTR for a better understanding of the processes of urethral masculinization.

    UR - http://www.scopus.com/inward/record.url?scp=84956919106&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=84956919106&partnerID=8YFLogxK

    U2 - 10.1210/en.2015-1586

    DO - 10.1210/en.2015-1586

    M3 - Article

    VL - 157

    SP - 844

    EP - 857

    JO - Endocrinology

    JF - Endocrinology

    SN - 0013-7227

    IS - 2

    ER -