Aneuploid plants derived from crosses with triploid grapes through immature seed culture and subsequent embryo culture

S. M. Park, Michikazu Hiramatsu, Akira Wakana

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Through immature seed culture and subsequent embryo culture, aneuploid plants were derived from various crosses among 184 different triploid hybrid grape vines. In self-pollinations of the 184 vines, 0 to 1.6% of flowers produced immature seeds. In 16 reciprocal crosses between diploid and triploid and between tetraploid and triploid grapes, 0 to 23.0% of flowers produced immature seeds. The immature seeds excised 30-50 days after pollination were cultured for three months on Nitsch and Nitsch medium supplemented with L-glutamine, L-serine, L-cysteine and casein hydrolysate. Embryos developed within the cultured immature seeds were subcultured onto germination medium consisting of MS medium with 1 μM BA. Thirty-four of 137 embryos from 458 immature seeds germinated. Five of the 34 embryos grew normally. The five recovered plants were aneuploids with chromosome numbers from 51 to 59. The rates of embryo and plant recovery were different in different crosses with triploid grapes.

Original languageEnglish
Pages (from-to)125-133
Number of pages9
JournalPlant Cell, Tissue and Organ Culture
Volume59
Issue number2
DOIs
Publication statusPublished - Dec 1 1999

All Science Journal Classification (ASJC) codes

  • Horticulture

Fingerprint Dive into the research topics of 'Aneuploid plants derived from crosses with triploid grapes through immature seed culture and subsequent embryo culture'. Together they form a unique fingerprint.

  • Cite this