Anisotropic Incorporation of Lipid-Anchored Myoglobin into a Phospholipid Bilayer Membrane

Itaru Hamachi, Kentaro Nakamura, Akio Fujita, Toyoki Kunitake

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Directionally controlled anchoring of myoglobin, a water-soluble globular protein, on a dipalmitoylphos-phatidylcholine (DPPC) bilayer has been successfully conducted. Lipid-anchored myoglobin was obtained from a monoalkylated heme derivative and apomyoglobin. It was indicated by gel filtration and ultrafiltration studies that the lipid-anchored myoglobin Mb(1a), but not native myoglobin and Mb(2a), is bound to the DPPC bilayer membrane in aqueous dispersion. A cast film of the phospholipid and the anchored myoglobin displayed anisotropic ESR signals, which depend on the disposition of the cast film in the magnetic field. These results suggest that myoglobin molecules are placed on the lipid bilayer in a fixed orientation by inserting the anchor alkyl chain into the bilayer. The ESR anisotropy was not observed without the anchor.

Original languageEnglish
Pages (from-to)4966-4970
Number of pages5
JournalJournal of the American Chemical Society
Volume115
Issue number12
DOIs
Publication statusPublished - Jun 1 1993

Fingerprint

Myoglobin
Phospholipids
Anchors
Lipids
Paramagnetic resonance
Membranes
Lipid bilayers
Ultrafiltration
Anisotropy
Gels
Magnetic fields
Derivatives
Proteins
Molecules
Water
Lipid Bilayers
Magnetic Fields
Heme
Gel Chromatography

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

Cite this

Anisotropic Incorporation of Lipid-Anchored Myoglobin into a Phospholipid Bilayer Membrane. / Hamachi, Itaru; Nakamura, Kentaro; Fujita, Akio; Kunitake, Toyoki.

In: Journal of the American Chemical Society, Vol. 115, No. 12, 01.06.1993, p. 4966-4970.

Research output: Contribution to journalArticle

Hamachi, Itaru ; Nakamura, Kentaro ; Fujita, Akio ; Kunitake, Toyoki. / Anisotropic Incorporation of Lipid-Anchored Myoglobin into a Phospholipid Bilayer Membrane. In: Journal of the American Chemical Society. 1993 ; Vol. 115, No. 12. pp. 4966-4970.
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