Anomalous Scaling of Gene Expression in Confined Cell-Free Reactions

Ryota Sakamoto, Vincent Noireaux, Yusuke Maeda

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Cellular surface breaks the symmetry of molecular diffusion across membrane. Here, we study how steric interactions between the surface and the bulk of cell-sized emulsion droplets alters gene expression emulated by a cell-free transcription/translation (TXTL) system. The concentration of synthesized reporter proteins in droplets of radius R shows an anomalous geometric scaling of R4 different from the expected size-dependence of R3. Given that TXTL becomes less efficient at thin surface layer, a mathematical model explains the anomalous size-dependence found in experiment. The surface of cell-sized compartment can thus play a regulatory role for cell-free gene expression.

Original languageEnglish
Article number7364
JournalScientific reports
Volume8
Issue number1
DOIs
Publication statusPublished - Dec 1 2018

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Gene Expression
Emulsions
Theoretical Models
Membranes
Proteins

All Science Journal Classification (ASJC) codes

  • General

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Anomalous Scaling of Gene Expression in Confined Cell-Free Reactions. / Sakamoto, Ryota; Noireaux, Vincent; Maeda, Yusuke.

In: Scientific reports, Vol. 8, No. 1, 7364, 01.12.2018.

Research output: Contribution to journalArticle

Sakamoto, Ryota ; Noireaux, Vincent ; Maeda, Yusuke. / Anomalous Scaling of Gene Expression in Confined Cell-Free Reactions. In: Scientific reports. 2018 ; Vol. 8, No. 1.
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