Anti-androgen flutamide affects gonadal development and reproduction in medaka (Oryzias latipes)

Ik Joon Kang, Takeshi Hano, Yuji Oshima, Hirofumi Yokota, Yukinari Tsuruda, Yohei Shimasaki, Tsuneo Honjo

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Abstract

The effects of an anti-androgenic chemical on the reproduction of medaka (Oryzias latipes) were examined. Six mating pairs of medaka were exposed to flutamide (FLU) at measured concentrations of 0.101, 0.202, 0.397, 0.787, or 1.56 mg/L for 3 weeks. During the exposure period, one female died in the 1.56-mg/L treatment group, but no lesions or pathological abnormalities were observed. The fecundity and fertility of paired medaka were significantly decreased at 1.56 mg/L compared with those of the controls. Histological examination showed intersex gonads (testis-ova) in males exposed to FLU at 0.202, 0.397, and 0.787 mg/L. However, concentrations of hepatic vitellogenin (Vtg) in both sexes were not statistically different from those in the controls. These results demonstrate that FLU affected gonadal development in male medaka and reproductive capacity in paired medaka. This is the first report of testis-ova in male medaka exposed to FLU.

Original languageEnglish
Pages (from-to)S253-S257
JournalMarine Environmental Research
Volume62
Issue numberSUPPL. 1
DOIs
Publication statusPublished - Jun 19 2006

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All Science Journal Classification (ASJC) codes

  • Oceanography
  • Aquatic Science
  • Pollution

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