Anti-CXCR3 staining is useful for detecting human cutaneous and mucosal mast cells

Noriko Fukiwake, Yoichi Moroi, Shinichi Imafuku, Teiichi Masuda, Hisashi Kokuba, Masutaka Furue, Kazunori Urabe

Research output: Contribution to journalArticle

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Abstract

Human synovial mast cells (MC) can be immunolabelled with antihuman CXCR3 antibody (Ab) (clone 49801). We have investigated whether cutaneous and mucosal MC are stained with anti-CXCR3 Ab in paraffin-embedded sections. Immunohistochemical staining and immunofluorescence double staining assays were performed with anti-CXCR3, anti-tryptase, and anti-chymase Ab using normal skin, psoriatic skin lesions, and normal colon. When compared with tryptase and chymase staining, 100% of the cutaneous and 98% of the mucosal MC were positive for CXCR3. Anti-CXCR3 staining is a useful marker for human cutaneous and mucosal MC in paraffin-embedded sections.

Original languageEnglish
Pages (from-to)326-330
Number of pages5
JournalJournal of Dermatology
Volume33
Issue number5
DOIs
Publication statusPublished - May 1 2006

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Mast Cells
Staining and Labeling
Skin
Chymases
Tryptases
Paraffin
Anti-Idiotypic Antibodies
Fluorescent Antibody Technique
Colon
Clone Cells
Antibodies

All Science Journal Classification (ASJC) codes

  • Dermatology

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Anti-CXCR3 staining is useful for detecting human cutaneous and mucosal mast cells. / Fukiwake, Noriko; Moroi, Yoichi; Imafuku, Shinichi; Masuda, Teiichi; Kokuba, Hisashi; Furue, Masutaka; Urabe, Kazunori.

In: Journal of Dermatology, Vol. 33, No. 5, 01.05.2006, p. 326-330.

Research output: Contribution to journalArticle

Fukiwake, N, Moroi, Y, Imafuku, S, Masuda, T, Kokuba, H, Furue, M & Urabe, K 2006, 'Anti-CXCR3 staining is useful for detecting human cutaneous and mucosal mast cells', Journal of Dermatology, vol. 33, no. 5, pp. 326-330. https://doi.org/10.1111/j.1346-8138.2006.00077.x
Fukiwake, Noriko ; Moroi, Yoichi ; Imafuku, Shinichi ; Masuda, Teiichi ; Kokuba, Hisashi ; Furue, Masutaka ; Urabe, Kazunori. / Anti-CXCR3 staining is useful for detecting human cutaneous and mucosal mast cells. In: Journal of Dermatology. 2006 ; Vol. 33, No. 5. pp. 326-330.
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