Anti-inflammatory activity of a globular adiponectin function on RAW 264 cells stimulated by lipopolysaccharide from Aggregatibacter actinomycetemcomitans

Noriaki Kamio, Sumio Akifusa, Noboru Yamaguchi, Kazuaki Nonaka, Yoshihisa Yamashita

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Adiponectin is an adipokine with potent anti-inflammatory properties. We previously reported that a globular adiponectin (gAd) suppresses Aggregatibacter actinomycetemcomitans lipopolysaccharide-induced nuclear factor-κB activity, suggesting an anti-inflammatory effect of gAd. In this study, we investigated whether gAd is able to modulate the effect of A. actinomycetemcomitans lipopolysaccharide on cytokine induction in a murine macrophage cell line (RAW 264). The phosphorylation of p38 mitogen-activated protein kinase, c-Jun N-terminal kinase, extracellular signal-regulated kinase, and IκB kinase α/β and the degradation of IκB, which were induced by A. actinomycetemcomitans lipopolysaccharide intoxication, were clearly reduced in gAd-pretreated RAW 264 cells compared with the untreated cells. Expression levels of tumor necrosis factor (TNF)-α and interleukin-10 (IL-10) mRNA were assessed by real-time PCR. Cell-free supernatants were collected after 12 h of stimulation and analyzed by enzyme-linked immunosorbent assay for TNF-α and IL-10. Pretreatment with gAd significantly inhibited the A. actinomycetemcomitans lipopolysaccharide- induced TNF-α mRNA expression and protein secretion. In contrast, pretreatment with gAd significantly enhanced the A. actinomycetemcomitans lipopolysaccharide-induced IL-10 mRNA expression and protein secretion. These data suggest a mechanism for the anti-inflammatory activity of gAd in local inflammatory lesions, such as periodontitis.

Original languageEnglish
Pages (from-to)241-247
Number of pages7
JournalFEMS Immunology and Medical Microbiology
Volume56
Issue number3
DOIs
Publication statusPublished - Aug 1 2009

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Aggregatibacter actinomycetemcomitans
Adiponectin
Lipopolysaccharides
Anti-Inflammatory Agents
Interleukin-10
Tumor Necrosis Factor-alpha
Messenger RNA
Adipokines
JNK Mitogen-Activated Protein Kinases
Periodontitis
Extracellular Signal-Regulated MAP Kinases
p38 Mitogen-Activated Protein Kinases
Real-Time Polymerase Chain Reaction
Proteins
Phosphotransferases
Enzyme-Linked Immunosorbent Assay
Macrophages
Phosphorylation
Cytokines
Cell Line

All Science Journal Classification (ASJC) codes

  • Immunology
  • Immunology and Allergy
  • Infectious Diseases
  • Microbiology (medical)
  • Microbiology

Cite this

Anti-inflammatory activity of a globular adiponectin function on RAW 264 cells stimulated by lipopolysaccharide from Aggregatibacter actinomycetemcomitans. / Kamio, Noriaki; Akifusa, Sumio; Yamaguchi, Noboru; Nonaka, Kazuaki; Yamashita, Yoshihisa.

In: FEMS Immunology and Medical Microbiology, Vol. 56, No. 3, 01.08.2009, p. 241-247.

Research output: Contribution to journalArticle

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