Anti-inflammatory/anti-amyloidogenic effects of plasmalogens in lipopolysaccharide-induced neuroinflammation in adult mice

Masataka Ifuku, Toshihiko Katafuchi, Shiro Mawatari, Mami Noda, Kiyotaka Miake, Masaaki Sugiyama, Takehiko Fujino

Research output: Contribution to journalArticle

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Abstract

Background: Neuroinflammation involves the activation of glial cells in neurodegenerative diseases such as Alzheimer's disease (AD). Plasmalogens (Pls) are glycerophospholipids constituting cellular membranes and play significant roles in membrane fluidity and cellular processes such as vesicular fusion and signal transduction.Methods: In this study the preventive effects of Pls on systemic lipopolysaccharide (LPS)-induced neuroinflammation were investigated using immunohistochemistry, real-time PCR methods and analysis of brain glycerophospholipid levels in adult mice.Results: Intraperitoneal (i.p.) injections of LPS (250 μg/kg) for seven days resulted in increases in the number of Iba-1-positive microglia and glial fibrillary acidic protein (GFAP)-positive astrocytes in the prefrontal cortex (PFC) and hippocampus accompanied by the enhanced expression of IL-1β and TNF-α mRNAs. In addition, β-amyloid (Aβ3-16)-positive neurons appeared in the PFC and hippocampus of LPS-injected animals. The co-administration of Pls (i.p., 20 mg/kg) after daily LPS injections significantly attenuated both the activation of glial cells and the accumulation of Aβ proteins. Finally, the amount of Pls in the PFC and hippocampus decreased following the LPS injections and this reduction was suppressed by co-treatment with Pls.Conclusions: These findings suggest that Pls have anti-neuroinflammatory and anti-amyloidogenic effects, thereby indicating the preventive or therapeutic application of Pls against AD.

Original languageEnglish
Article number197
JournalJournal of neuroinflammation
Volume9
DOIs
Publication statusPublished - Aug 13 2012

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Plasmalogens
Lipopolysaccharides
Anti-Inflammatory Agents
Prefrontal Cortex
Glycerophospholipids
Hippocampus
Neuroglia
Alzheimer Disease
Injections
Membrane Fluidity
Glial Fibrillary Acidic Protein
Microglia
Intraperitoneal Injections
Interleukin-1
Amyloid
Astrocytes
Neurodegenerative Diseases
Real-Time Polymerase Chain Reaction
Signal Transduction
Immunohistochemistry

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Immunology
  • Neurology
  • Cellular and Molecular Neuroscience

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Anti-inflammatory/anti-amyloidogenic effects of plasmalogens in lipopolysaccharide-induced neuroinflammation in adult mice. / Ifuku, Masataka; Katafuchi, Toshihiko; Mawatari, Shiro; Noda, Mami; Miake, Kiyotaka; Sugiyama, Masaaki; Fujino, Takehiko.

In: Journal of neuroinflammation, Vol. 9, 197, 13.08.2012.

Research output: Contribution to journalArticle

Ifuku, Masataka ; Katafuchi, Toshihiko ; Mawatari, Shiro ; Noda, Mami ; Miake, Kiyotaka ; Sugiyama, Masaaki ; Fujino, Takehiko. / Anti-inflammatory/anti-amyloidogenic effects of plasmalogens in lipopolysaccharide-induced neuroinflammation in adult mice. In: Journal of neuroinflammation. 2012 ; Vol. 9.
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