Anti-listeria activity of Pediococcus pentosaceus BCC 3772 and application as starter culture for Nham, a traditional fermented pork sausage

Yutthana Kingcha, Amonlaya Tosukhowong, Takeshi Zendo, Sittiruk Roytrakul, Plearnpis Luxananil, Kingeaw Chareonpornsook, Ruud Valyasevi, Kenji Sonomoto, Wonnop Visessanguan

Research output: Contribution to journalArticlepeer-review

48 Citations (Scopus)

Abstract

Lactic acid bacteria forming bacteriocin active against Listeria monocytogenes were screened for potential use in controlling growth of L. monocytogenes in Nham, a Thai traditional fermented pork sausage. Based on the spot-on-lawn assay, Pediococcus pentosaceus BCC 3772 was found to produce the highest anti-listeria activity. Through a series of chromatographic techniques, Edman N-terminal sequencing and mass spectrometry, this anti-listeria compound showed a complete homology to pediocin PA-1/AcH. Inoculation of P. pentosaceus BCC 3772 in Nham caused a significant decrease of 3.2 log in population of spiked L. monocytogenes within 18-24 h of fermentation, in comparison with the initial count with no significant changes in sensory properties and consumer acceptability on the overall characteristics of the final fermented Nham products. These results indicate that the P. pentosaceus BCC 3772 should be useful as a functional starter culture for control of L. monocytogenes without compromising the unique quality of Nham.

Original languageEnglish
Pages (from-to)190-196
Number of pages7
JournalFood Control
Volume25
Issue number1
DOIs
Publication statusPublished - May 1 2012

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Food Science

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