Anti-miroestrol polyclonal antibodies: A comparison of immunogen preparations used to obtain desired antibody properties

Tharita Kitisripanya, Kamonthip Jutathis, Chadathorn Inyai, Jukrapun Komaikul, Orapin Udomsin, Gorawit Yusakul, Hiroyuki Tanaka, Waraporn Putalun

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Immunogen quality is one important factor that contributes to desirable antibody characteristics. Highly specific antibodies against miroestrol can be used to develop a quality control immunoassay for Pueraria candollei products. In this study, we investigated how various immunogen preparations affect antibody properties. The results show that immunogen prepared using the Mannich reaction provides antibodies with higher specificity and sensitivity against miroestrol than immunogen prepared with the periodate reaction. The results suggest the Mannich reaction maintains the original structure of miroestrol and generates useful antibodies for developing immunoassays.

Original languageEnglish
Pages (from-to)296-299
Number of pages4
JournalJournal of Natural Medicines
Volume70
Issue number2
DOIs
Publication statusPublished - Apr 1 2016

All Science Journal Classification (ASJC) codes

  • Pharmaceutical Science
  • Drug Discovery
  • Complementary and alternative medicine
  • Organic Chemistry

Fingerprint Dive into the research topics of 'Anti-miroestrol polyclonal antibodies: A comparison of immunogen preparations used to obtain desired antibody properties'. Together they form a unique fingerprint.

  • Cite this

    Kitisripanya, T., Jutathis, K., Inyai, C., Komaikul, J., Udomsin, O., Yusakul, G., Tanaka, H., & Putalun, W. (2016). Anti-miroestrol polyclonal antibodies: A comparison of immunogen preparations used to obtain desired antibody properties. Journal of Natural Medicines, 70(2), 296-299. https://doi.org/10.1007/s11418-015-0949-x