Anti-patched-1 antibodies suppress hedgehog signaling pathway and pancreatic cancer proliferation

Masafumi Nakamura, Makoto Kubo, Kosuke Yanai, Yoshiko Mikami, Mio Ikebe, Shuntaro Nagai, Koji Yamaguchi, Masao Tanaka, Mitsuo Katano

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23 Citations (Scopus)

Abstract

Background: The hedgehog (Hh) signaling pathway is aberrantly activated in many human carcinomas including pancreatic cancer and regulates tumor cell growth. Overproduction of sonic hedgehog (Shh), a ligand of the Hh signaling pathway, increases the Hh signaling activity through transmitting the signal to patched-1 (Ptch1), the receptor of the Hh signaling pathway. Materials and Methods: α-Ptch1 antibodies were raised against an oligo-peptide, designed according to the Ptch1 amino-acid sequence. The specificity of α-Ptch1 was examined by immunoblotting and immuno-fluorescence, and biological effects were detected by RT-PCR and cell proliferation assay using two pancreatic cancer cell lines, Panc1 and SUIT-2. Results: α-Ptch1 recognized a 160 kDa protein as shown by immunoblotting and cell surface staining of pancreatic cancer cells. Incubation with α-Ptch1 suppressed Hh signaling activity and proliferation of pancreatic cancer cells. Conclusion: These results provide a new strategy for controling Hh dependent development of pancreatic cancer and other Hh related carcinomas.

Original languageEnglish
Pages (from-to)3743-3747
Number of pages5
JournalAnticancer research
Volume27
Issue number6 A
Publication statusPublished - Nov 2007

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

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    Nakamura, M., Kubo, M., Yanai, K., Mikami, Y., Ikebe, M., Nagai, S., Yamaguchi, K., Tanaka, M., & Katano, M. (2007). Anti-patched-1 antibodies suppress hedgehog signaling pathway and pancreatic cancer proliferation. Anticancer research, 27(6 A), 3743-3747.