Anti-peptide antibody production elicited by in vitro immunization of human peripheral blood mononuclear cells

Takashi Tamura, Kosuke Tomimatsu, Yoshinori Katakura, Makiko Yamashita, Shin Ei Matsumoto, Yoshihiro Aiba, Yeon Suk Jung, Yoshiichi Abe, Tsukasa Fujiki, Kiichiro Teruya, Sanetaka Shirahata

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Human monoclonal antibodies have great potential for use in the treatment of various diseases. We have established an in vitro immunization protocol for inducing antigen-specific antibody production from human peripheral blood mononuclear cells (PBMCs). In the in vitro immunization protocol, PBMCs are pretreated with L-leucyl-L-leucine methyl ester (LLME) to remove suppressive cells, and are sensitized and cultured with a soluble antigen in the presence of IL-2, IL-4 and muramyl dipeptide for 8 d, and then an antigen-specific antibody is produced. In this study, we examined the novel possibility of an in vitro immunization protocol, specifically, whether LLME-treated PBMCs can be sensitized with a peptide antigen to produce an anti-peptide antibody. The results indicate that antigen-specific immune responses were elicited by a peptide antigen derived from rice allergen, a cholera toxin B subunit, and TNF-α as a sensitizing antigen in in vitro immunization. These results suggest that the in vitro immunization protocol is applicable in the generation of an anti-peptide antibody against various antigens, including food allergens, foreign antigens, and self-antigens.

Original languageEnglish
Pages (from-to)2871-2875
Number of pages5
JournalBioscience, Biotechnology and Biochemistry
Volume71
Issue number12
DOIs
Publication statusPublished - Dec 1 2007

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Immunization
Antibody Formation
Anti-Idiotypic Antibodies
Blood Cells
Blood
Antigens
Peptides
Antibodies
Allergens
leucylleucine
Acetylmuramyl-Alanyl-Isoglutamine
In Vitro Techniques
Cholera Toxin
Histocompatibility Antigens Class II
Autoantigens
Leucine
Interleukin-4
Interleukin-2
Cultured Cells
Monoclonal Antibodies

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

Cite this

Anti-peptide antibody production elicited by in vitro immunization of human peripheral blood mononuclear cells. / Tamura, Takashi; Tomimatsu, Kosuke; Katakura, Yoshinori; Yamashita, Makiko; Matsumoto, Shin Ei; Aiba, Yoshihiro; Jung, Yeon Suk; Abe, Yoshiichi; Fujiki, Tsukasa; Teruya, Kiichiro; Shirahata, Sanetaka.

In: Bioscience, Biotechnology and Biochemistry, Vol. 71, No. 12, 01.12.2007, p. 2871-2875.

Research output: Contribution to journalArticle

Tamura, T, Tomimatsu, K, Katakura, Y, Yamashita, M, Matsumoto, SE, Aiba, Y, Jung, YS, Abe, Y, Fujiki, T, Teruya, K & Shirahata, S 2007, 'Anti-peptide antibody production elicited by in vitro immunization of human peripheral blood mononuclear cells', Bioscience, Biotechnology and Biochemistry, vol. 71, no. 12, pp. 2871-2875. https://doi.org/10.1271/bbb.60460
Tamura, Takashi ; Tomimatsu, Kosuke ; Katakura, Yoshinori ; Yamashita, Makiko ; Matsumoto, Shin Ei ; Aiba, Yoshihiro ; Jung, Yeon Suk ; Abe, Yoshiichi ; Fujiki, Tsukasa ; Teruya, Kiichiro ; Shirahata, Sanetaka. / Anti-peptide antibody production elicited by in vitro immunization of human peripheral blood mononuclear cells. In: Bioscience, Biotechnology and Biochemistry. 2007 ; Vol. 71, No. 12. pp. 2871-2875.
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