Anti-solasodine glycoside single-chain Fv antibody stimulates biosynthesis of solasodine glycoside in plants

W. Putalun, F. Taura, W. Qing, H. Matsushita, H. Tanaka, Y. Shoyama

Research output: Contribution to journalArticlepeer-review

35 Citations (Scopus)

Abstract

We constructed a recombinant antibody fragment - single chain fragment-variable (scFv) antibody -derived from hybridoma cell lines to control the concentration of solasodine glycosides in hairy root cultures of Solanum khasianum transformed by the anti-solamargine (As)-scFv gene. The properties of the As-scFv protein expressed in Escherichia coli were almost identical to those of the parent monoclonal antibody (MAb). Up to 220 ng recombinant As-scFv was expressed per milligram of soluble protein in transgenic hairy root cultures of S. khasianum. The concentration of solasodine glycosides was 2.3-fold higher in the transgenic than in the wild-type hairy root, as reflected by the soluble As-scFv level and antigen binding activities. These results suggested that the scFv antibody expressed in transgenic hairy roots controlled the antigen level, thus representing a novel plant breeding methodology that can produce secondary metabolites.

Original languageEnglish
Pages (from-to)344-349
Number of pages6
JournalPlant Cell Reports
Volume22
Issue number5
DOIs
Publication statusPublished - Dec 2003

All Science Journal Classification (ASJC) codes

  • Agronomy and Crop Science
  • Plant Science

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