Abstract
OBJECTIVES: To determine whether heat-shock protein (HSP) 60-recognizing CD4+ T cells show antitumour activity against renal carcinoma (RENCA) cells, as HSP is highly expressed by tumour cells and induced in cells by various stresses, including transformation. MATERIALS AND METHODS: RENCA, a renal cortical adenocarcinoma cell line of BALB/c origin, was used. Expression of major histocompatibility complex (MHC) class I, class II and HSP-60 on RENCA tumour cells was analysed by flow cytometry. BASL1.1, an autoreactive T-helper type 1 type CD4+ T cell clone established by us, and that recognises HSP-60, was also used for a tumour-neutralising assay. RESULTS: The RENCA cells were negative for MHC class II, but expressed intracellular HSP-60. In the tumour-neutralising assay, BASL1.1 cells significantly suppressed the in vivo growth of RENCA cells. Three of five mice rejected RENCA cells when co-inoculated with BASL1.1 cells. CONCLUSIONS: These results indicate that HSP-60-recognizing CD4+ T cells have the potential to eliminate renal cell carcinoma in vivo and that the eliciting of an anti-self T cell response at the tumour site can lead to regression of renal cancer.
| Original language | English |
|---|---|
| Pages (from-to) | 421-424 |
| Number of pages | 4 |
| Journal | BJU international |
| Volume | 95 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Feb 1 2005 |
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All Science Journal Classification (ASJC) codes
- Urology
Cite this
Anti-tumour activity of heat-shock protein 60-recognizing CD4+ T cells against syngeneic murine renal cell carcinoma. / Eto, Masatoshi; Harada, Mamoru; Tatsugami, Katsunori; Harano, Masahiko; Koga, Hirofumi; Matsuzaki, Goro; Naito, Seiji.
In: BJU international, Vol. 95, No. 3, 01.02.2005, p. 421-424.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Anti-tumour activity of heat-shock protein 60-recognizing CD4+ T cells against syngeneic murine renal cell carcinoma
AU - Eto, Masatoshi
AU - Harada, Mamoru
AU - Tatsugami, Katsunori
AU - Harano, Masahiko
AU - Koga, Hirofumi
AU - Matsuzaki, Goro
AU - Naito, Seiji
PY - 2005/2/1
Y1 - 2005/2/1
N2 - OBJECTIVES: To determine whether heat-shock protein (HSP) 60-recognizing CD4+ T cells show antitumour activity against renal carcinoma (RENCA) cells, as HSP is highly expressed by tumour cells and induced in cells by various stresses, including transformation. MATERIALS AND METHODS: RENCA, a renal cortical adenocarcinoma cell line of BALB/c origin, was used. Expression of major histocompatibility complex (MHC) class I, class II and HSP-60 on RENCA tumour cells was analysed by flow cytometry. BASL1.1, an autoreactive T-helper type 1 type CD4+ T cell clone established by us, and that recognises HSP-60, was also used for a tumour-neutralising assay. RESULTS: The RENCA cells were negative for MHC class II, but expressed intracellular HSP-60. In the tumour-neutralising assay, BASL1.1 cells significantly suppressed the in vivo growth of RENCA cells. Three of five mice rejected RENCA cells when co-inoculated with BASL1.1 cells. CONCLUSIONS: These results indicate that HSP-60-recognizing CD4+ T cells have the potential to eliminate renal cell carcinoma in vivo and that the eliciting of an anti-self T cell response at the tumour site can lead to regression of renal cancer.
AB - OBJECTIVES: To determine whether heat-shock protein (HSP) 60-recognizing CD4+ T cells show antitumour activity against renal carcinoma (RENCA) cells, as HSP is highly expressed by tumour cells and induced in cells by various stresses, including transformation. MATERIALS AND METHODS: RENCA, a renal cortical adenocarcinoma cell line of BALB/c origin, was used. Expression of major histocompatibility complex (MHC) class I, class II and HSP-60 on RENCA tumour cells was analysed by flow cytometry. BASL1.1, an autoreactive T-helper type 1 type CD4+ T cell clone established by us, and that recognises HSP-60, was also used for a tumour-neutralising assay. RESULTS: The RENCA cells were negative for MHC class II, but expressed intracellular HSP-60. In the tumour-neutralising assay, BASL1.1 cells significantly suppressed the in vivo growth of RENCA cells. Three of five mice rejected RENCA cells when co-inoculated with BASL1.1 cells. CONCLUSIONS: These results indicate that HSP-60-recognizing CD4+ T cells have the potential to eliminate renal cell carcinoma in vivo and that the eliciting of an anti-self T cell response at the tumour site can lead to regression of renal cancer.
UR - http://www.scopus.com/inward/record.url?scp=14144250442&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=14144250442&partnerID=8YFLogxK
U2 - 10.1111/j.1464-410X.2005.05313.x
DO - 10.1111/j.1464-410X.2005.05313.x
M3 - Article
C2 - 15679807
AN - SCOPUS:14144250442
VL - 95
SP - 421
EP - 424
JO - BJU International
JF - BJU International
SN - 1464-4096
IS - 3
ER -