TY - JOUR
T1 - Antiallergic Activity of the Wild Mushrooms of Nepal and the Pure Compound Hispidin
AU - Tamrakar, Sonam
AU - Fukami, Katsuya
AU - Parajuli, Gopal Prasad
AU - Shimizu, Kuniyoshi
N1 - Funding Information:
The authors thank the Nepal Agricultural Research Council (NARC) for providing the mushroom samples. The support of the Ministry of Education, Culture, Sports, Science and Technology of Japan (MEXT) in the form of PhD scholarship is greatly acknowledged. This work was supported by KAKENHI Grant Number 26304047.
Publisher Copyright:
© 2019, Mary Ann Liebert, Inc.
PY - 2019/2
Y1 - 2019/2
N2 - In the present study, ethanol extracts of 90 wild mushroom samples from Nepal, and the pure compound hispidin, were screened for their ability to inhibit β-hexosaminidase release (BHR) from rat basophilic leukemia-2H3 cells. Simultaneously, the toxicity of the extracts toward the cells was also determined, using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Samples belonging to the groups Hymenochaetales and Polyporales showed promising anti-allergic activity, with Phellinus adamantinus and Ganoderma lingzhi 3 allowing a mere 19.4% and 16.7% BHR, respectively, without any cell cytotoxicity. Moreover, the 50% inhibitory concentration (IC 50 ) values for Inonotus clemensiae and P. adamantinus were determined to be 51.24 and 50.65 μg/mL, respectively; whereas hispidin, the major bioactive compound in I. clemensiae showed an IC 50 value of 82.47 μg/mL. These findings are crucial in underscoring the medicinal value of the wild mushrooms of Nepal, as a source of strong antiallergic agents.
AB - In the present study, ethanol extracts of 90 wild mushroom samples from Nepal, and the pure compound hispidin, were screened for their ability to inhibit β-hexosaminidase release (BHR) from rat basophilic leukemia-2H3 cells. Simultaneously, the toxicity of the extracts toward the cells was also determined, using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Samples belonging to the groups Hymenochaetales and Polyporales showed promising anti-allergic activity, with Phellinus adamantinus and Ganoderma lingzhi 3 allowing a mere 19.4% and 16.7% BHR, respectively, without any cell cytotoxicity. Moreover, the 50% inhibitory concentration (IC 50 ) values for Inonotus clemensiae and P. adamantinus were determined to be 51.24 and 50.65 μg/mL, respectively; whereas hispidin, the major bioactive compound in I. clemensiae showed an IC 50 value of 82.47 μg/mL. These findings are crucial in underscoring the medicinal value of the wild mushrooms of Nepal, as a source of strong antiallergic agents.
UR - http://www.scopus.com/inward/record.url?scp=85061279698&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85061279698&partnerID=8YFLogxK
U2 - 10.1089/jmf.2018.4267
DO - 10.1089/jmf.2018.4267
M3 - Article
C2 - 30596532
AN - SCOPUS:85061279698
SN - 1096-620X
VL - 22
SP - 225
EP - 227
JO - Journal of Medicinal Food
JF - Journal of Medicinal Food
IS - 2
ER -