TY - JOUR
T1 - Antibacterial activity of Nepalese wild mushrooms against Staphylococcus aureus and Propionibacterium acnes
AU - Tamrakar, Sonam
AU - Nishida, Marina
AU - Amen, Yhiya
AU - Tran, Hai Bang
AU - Suhara, Hiroto
AU - Fukami, Katsuya
AU - Parajuli, Gopal Prasad
AU - Shimizu, Kuniyoshi
N1 - Funding Information:
The authors would like to thank the Center for Advanced Instrumental and Educational Support, Faculty of Agriculture, Kyushu University for supporting the LCMS-IT-TOF analysis; and the Network Joint Research Center for Materials and Devices, Kyushu University for the NMR analysis. We are thankful to Nepal Agricultural Research Council for providing the mushroom samples. The support of the Ministry of Education, Culture, Sports, Science and Technology of Japan (MEXT) in the form of PhD Scholarship is greatly acknowledged. This work was supported by KAKENHI Grant Number 26304047.
Publisher Copyright:
© 2017, The Japan Wood Research Society.
PY - 2017/8/1
Y1 - 2017/8/1
N2 - The present study aims to reveal the antibacterial potential of the wild mushrooms of Nepal. Despite the recognition of the medicinal potential of the natural resources in this country, a systematic study on the bioactivities of the wild mushrooms is still lacking. Therefore, in an attempt to fill this gap, ethanol extracts of 90 Nepalese wild mushroom samples were tested for antibacterial activity against Staphylococcus aureus and Propionibacterium acnes. Staphylococcus aureus was comparatively more susceptible with Inonotus clemensiae exhibiting the least minimum bactericidal concentration (MBC) of 100 μg/mL. The major compound in I. clemensiae was identified to be hispidin using high resolution liquid chromatography–electron spray ionization mass spectrometry (LC–ESI-MS) and nuclear magnetic resonance (NMR) analyses. Furthermore, the minimum inhibitory concentration (MIC) and MBC values of hispidin were determined to be 25 and 100 µg/mL for S. aureus and P. acnes, respectively. These findings show that the Nepalese wild mushrooms have the potential to be a novel addition to the functional ingredients industry due to their strong antibacterial potential.
AB - The present study aims to reveal the antibacterial potential of the wild mushrooms of Nepal. Despite the recognition of the medicinal potential of the natural resources in this country, a systematic study on the bioactivities of the wild mushrooms is still lacking. Therefore, in an attempt to fill this gap, ethanol extracts of 90 Nepalese wild mushroom samples were tested for antibacterial activity against Staphylococcus aureus and Propionibacterium acnes. Staphylococcus aureus was comparatively more susceptible with Inonotus clemensiae exhibiting the least minimum bactericidal concentration (MBC) of 100 μg/mL. The major compound in I. clemensiae was identified to be hispidin using high resolution liquid chromatography–electron spray ionization mass spectrometry (LC–ESI-MS) and nuclear magnetic resonance (NMR) analyses. Furthermore, the minimum inhibitory concentration (MIC) and MBC values of hispidin were determined to be 25 and 100 µg/mL for S. aureus and P. acnes, respectively. These findings show that the Nepalese wild mushrooms have the potential to be a novel addition to the functional ingredients industry due to their strong antibacterial potential.
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U2 - 10.1007/s10086-017-1636-1
DO - 10.1007/s10086-017-1636-1
M3 - Article
AN - SCOPUS:85020191283
SN - 1435-0211
VL - 63
SP - 379
EP - 387
JO - Journal of Wood Science
JF - Journal of Wood Science
IS - 4
ER -