Antibacterial-agent-immobilized gelatin hydrogel as a 3d scaffold for natural and bioengineered tissues

Tuyajargal Iimaa, Takaaki Hirayama, Nana Shirakigawa, Daisuke Imai, Takanobu Yamao, Yo Ichi Yamashita, Hideo Baba, Hiroyuki Ijima

Research output: Contribution to journalArticle

Abstract

Hydrogels and their medical applications in tissue engineering have been widely studied due to their three-dimensional network structure, biocompatibility, and cell adhesion. However, the development of an artificial bile duct to replace the recipient’s tissue is still desired. Some challenges remain in the tissue engineering field, such as infection due to residual artifacts. In other words, at present, there are no established technologies for bile duct reconstruction as strength and biocompatibility problems. Therefore, this study investigated hydrogel as an artificial bile duct base material that can replace tissue without any risk of infectious diseases. First, an antibacterial agent (ABA), Finibax (an ABA used for the clinical treatment of biliary tract infection), was immobilized in gelatin using a crosslinking agent, and the antibacterial properties of the gel and its sustainability were tested. Furthermore, the immobilized amount and the improvement of the proliferation of the human umbilical vein endothelial cells (HUVECs) were cultured as the ABA-Gelatin hydrogel was introduced to prepare a 3D scaffold. Finally, we performed hematoxylin and eosin (H&E) staining after subcutaneous implantation in the rat. Overall, the ABA-Gelatin hydrogel was found to be viable for use in hydrogel applications for tissue engineering due to its good bactericidal ability, cell adhesion, and proliferation, as well as having no cytotoxicity to cells.

Original languageEnglish
Article number32
JournalGels
Volume5
Issue number2
DOIs
Publication statusPublished - Jun 1 2019

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Bactericides
Hydrogel
Gelatin
Hydrogels
Scaffolds
Tissue
Anti-Bacterial Agents
Tissue engineering
Ducts
Cell adhesion
Biocompatibility
Endothelial cells
Cell proliferation
Medical applications
Hematoxylin
Eosine Yellowish-(YS)
Cytotoxicity
Crosslinking
Rats
Sustainable development

All Science Journal Classification (ASJC) codes

  • Organic Chemistry
  • Bioengineering
  • Biomaterials
  • Polymers and Plastics

Cite this

Antibacterial-agent-immobilized gelatin hydrogel as a 3d scaffold for natural and bioengineered tissues. / Iimaa, Tuyajargal; Hirayama, Takaaki; Shirakigawa, Nana; Imai, Daisuke; Yamao, Takanobu; Yamashita, Yo Ichi; Baba, Hideo; Ijima, Hiroyuki.

In: Gels, Vol. 5, No. 2, 32, 01.06.2019.

Research output: Contribution to journalArticle

Iimaa, T, Hirayama, T, Shirakigawa, N, Imai, D, Yamao, T, Yamashita, YI, Baba, H & Ijima, H 2019, 'Antibacterial-agent-immobilized gelatin hydrogel as a 3d scaffold for natural and bioengineered tissues', Gels, vol. 5, no. 2, 32. https://doi.org/10.3390/gels5020032
Iimaa T, Hirayama T, Shirakigawa N, Imai D, Yamao T, Yamashita YI et al. Antibacterial-agent-immobilized gelatin hydrogel as a 3d scaffold for natural and bioengineered tissues. Gels. 2019 Jun 1;5(2). 32. https://doi.org/10.3390/gels5020032
Iimaa, Tuyajargal ; Hirayama, Takaaki ; Shirakigawa, Nana ; Imai, Daisuke ; Yamao, Takanobu ; Yamashita, Yo Ichi ; Baba, Hideo ; Ijima, Hiroyuki. / Antibacterial-agent-immobilized gelatin hydrogel as a 3d scaffold for natural and bioengineered tissues. In: Gels. 2019 ; Vol. 5, No. 2.
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